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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
1C5-D9, monoclonal
Species reactivity:
human
Application:
—
Citations:
-
biological source
mouse
Quality Segment
antibody form
purified antibody
antibody product type
primary antibodies
clone
1C5-D9, monoclonal
form
liquid
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(ELISA
Immunoblotting (1-5 µg/mL)
Paraffin Sections (3-5 µg/mL))
isotype
IgG2a
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CLDN1(9076)
General description
Purified mouse monoclonal antibody. Recognizes the ~17 kDa CLDN1 protein.
Recognizes the ~17 kDa CLDN1 protein in PRC/PRF/5 and A431 cells and human small intestine and colon tissue.
This Anti-CLDN1 Mouse mAb (1C5-D9) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of CLDN1.
Immunogen
Full-length, recombinant, human CLDN1 (aa 1-212), expressed as a GST fusion protein
Application




ELISA (see comments)
Immunoblotting (1-5 g/ml)
Paraffin Sections (3-5 µg/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Negative Control
293T cells
293T cells
Positive Control
PRC/PRF/5 cells, A431 cells, Human small intestine tissue, human colon tissue
PRC/PRF/5 cells, A431 cells, Human small intestine tissue, human colon tissue
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Guevin C, et al. 2009. Antiviral Res.84, 14.
Sainz, B., Jr, et al. 2009. Virol. J.6, 103.
Stamataki Z, et al. 2009. Blood113 585.
Farquhar MJ, et al. 2008. J. Virol.82 8797.
Sainz, B., Jr, et al. 2009. Virol. J.6, 103.
Stamataki Z, et al. 2009. Blood113 585.
Farquhar MJ, et al. 2008. J. Virol.82 8797.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)
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Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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