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CBL1512Z

Sigma-Aldrich

Anti-Integrin αM [CD11b] Antibody, clone OX-42

clone OX-42, Chemicon®, from mouse

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Synonym(s):
CD11b
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

OX-42, monoclonal

species reactivity

rat

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable

isotype

IgG2a

NCBI accession no.

NM_000632.3

UniProt accession no.

P11215

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

rat ... Itgam(25021)

Related Categories

Specificity

This antibody recognizes most macrophages (including resident peritoneal and activated macrophages), Kupffer cells, but only about 35% of alveolar macrophages. The antibody also labels dendritic cells extensively, granulocytes and cells with the morphology of microglia in brain. The antibody precipitates three polypeptides of 150, 103 and 95 KDa. The antibody inhibits complement mediated rosettes and is probably the rat equivalent of the human receptor for IC3b called CR3. FUSION PARTNER: NSO myeloma cell line.

Immunogen

Rat peritoneal macrophages

Application

Detect Integrin αM [CD11b] using this Anti-Integrin αM CD11b Antibody, clone OX-42 validated for use in FC, IH, IH(P) & IP.
Flow cytometry

Immunohistochemical studies of rat fresh frozen tissue sections and paraffin-embedded tissue sections following either periodate-lysine-paraformaldehyde (PLP) fixation, or acetone. Works on very lightly PFA fixed, frozen tissues. (perfusion only 4% PFA 10-15′ no post-fix).

Immunoprecipitation: use rabbit anti-mouse or anti-mouse IgG bead for capture only. The use of protein A or protein G is not recommended.

Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
Integrins

Linkage

Replaces: MAB1405Z

Physical form

Format: Purified
The immunoglobulin was purified by protein G affinity chromatography and is presented as a liquid in phosphate buffered saline containing no preservatives.

Storage and Stability

Maintain in undiluted aliquots at -20°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Rosemary C Jones et al.
Nature protocols, 3(3), 388-397 (2008-03-08)
The goal of many current studies of neovascularization is to define the phenotype of vascular cell populations of different origins and to determine how such cells promote assembly of vascular channel. Here, we describe a protocol to immunophenotype vascular cells
Fabrice P Navarro et al.
Journal of neurochemistry, 105(1), 34-45 (2007-11-13)
Heparanase is an endo-beta-d-glucuronidase which specifically cleaves extracellular and cell surface heparan sulphates at intra-chain sites. Its enzymatic activity is strongly implicated in cell dissemination associated with tumor metastasis and inflammation. Indeed, heparanase gene is expressed in various tumors and
Andrea Beatriz Cragnolini et al.
Molecular and cellular neurosciences, 88, 240-248 (2018-02-15)
Astrocytes are a heterogeneous population of glial cells that react to brain insults through a process referred to as astrogliosis. Reactive astrocytes are characterized by an increase in proliferation, size, migration to the injured zone and release of a plethora
Hypertonic saline alleviates cerebral edema by inhibiting microglia-derived TNF-? and IL-1?-induced Na-K-Cl Cotransporter up-regulation.
Huang, LQ; Zhu, GF; Deng, YY; Jiang, WQ; Fang, M; Chen, CB; Cao, W; Wen, MY; Han, YL; Zeng, HK
Journal of Neuroinflammation null

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