Anti-Keratin K3/K76 Antibody, clone AE5

64 kDa

Rabbit corneal epithelial keratin

Anti-Keratin K3/K76 Antibody, clone AE5 is an antibody against Keratin K3/K76 for use in WB, IH, IH(P).

Immunohistochemistry on frozen sections: 1:50.

Immunohistochemistry on Paraffin-embedded sections: 1:50 on paraffin-embedded tissue sections fixed in 10% neutral buffered formalin or Fekete’s acid-alcohol-formalin following trypsin digestion antigen retrieval (Mikaelian et al., 2004).

Western blotting: 1:500.

Optimal working dilutions must be determined by the end user.

The antibody reacts with the 64 kDa polypeptide (cytokeratin 3; Keratin K3) of corneal epithelium and keratin K76 (cytokeratin K2p) of palate epithelium. As such this represents an excellent marker for studying corneal epithelial-type differentiation. Also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line.

Format: Purified

The monoclonal was purified by ion exchange chromatography is presented in phosphate buffered saline (pH 7.2) containing 0.09% sodium azide.

For use within 1 month of purchase store at 2°-8°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to one year.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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