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Merck
CN

CC095-M

Sigma-Aldrich

Mouse Laminin

from Engelbreth-Holm-Swarm murine sarcoma basement membrane, liquid, 1 mg/mL, suitable for cell culture

Synonym(s):

Purified Laminin derived for mouse

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75

Key Documents

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Product Name

Laminin, mouse purified, Laminins are the major noncollagenous constituent of basement membranes. This purified mouse laminin was isolated from Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a mouse tumor that produces large amounts of basement membranes.

biological source

mouse

Quality Level

100

form

liquid

manufacturer/tradename

Chemicon®

concentration

1 mg/mL

technique(s)

cell culture | mammalian: suitable

input

sample type: human embryonic stem cell(s)
sample type hematopoietic stem cell(s)
sample type epithelial cells
sample type: mouse embryonic stem cell(s)
sample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)
sample type neural stem cell(s)
sample type pancreatic stem cell(s)

NCBI accession no.

NM_002291.1

UniProt accession no.

P07942

shipped in

dry ice

Related Categories

General description

Laminin protein is a non-collagenous glycoprotein isolated from Engelbreth-Holm-Swarm (EHS) mouse sarcoma. A laminin protein molecule is composed of three disulfide-linked polypeptides that assemble to form an asymmetrical cross-shaped structure. Purified laminin mouse purified promotes the adhesion and growth of human carcinoma and sarcoma cells, human retinoblastoma cells, liver cells, mouse neuroblastoma cells, mouse embryonal carcinoma cells, and seems to be involved in the development of embryonal tissue. The optimal laminin concentration may vary with the cell type and has to be determined experimentally.

Application

The purified mouse laminin has been used as a coating substrate for culturing and maintaining mouse embryonic stem cell-derived neuronal cultures, providing a suitable surface for cell attachment and growth during in vitro experiments.

Biochem/physiol Actions

Laminin proteins are essential components of the basement membrane which are extracellular matrices holding cells and tissues together. They interact with receptors present in the plasma membrane of cells neighboring basement membranes. This interaction allows laminins to effectively regulate various cellular activities and signaling pathways.

Physical form

Purified from EHS sarcoma according to the method of Timpl, et al.
Purified protein. Liquid; in 0.05 M Tris-HCl, pH 7.4, containing 0.15 M NaCl.

No preservatives.

Preparation Note

Specificity: Molecular weight = 850 kD; 3 chains expressed as two major bands at 200 and 400 kD on SDS-PAGE.

Preparation: Prepared from Engelbreth-Holm-Swarm {EHS} sarcoma according to the method of Templ et al 1979 {http://www.jbc.org/cgi/reprint/254/19/9933.pdf} Briefly, tumor homogenates were subjected to NaCl extractions and centrifugations. Laminin was found in the 0.5M NaCl fraction. After subsequent salt gradients and further centrifugation steps, the laminin was dialyzed against 2M urea and passed over a column of DEAE, followed by molecular sieve chromatography and concentration by ultrafiltration.
Store at -20°C for up to six months.
Thaw product carefully at 2-8°C to avoid premature gelling, and do not refreeze. Once thawed. material should be stored at 2-8°C for up to 3 months.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The laminin family
Aumailley M
Journal of Separation Science, 7(1), 48-55 (2013)
G Siano et al.
Journal of molecular biology, 432(24), 166675-166675 (2020-10-16)
Neuronal hyperexcitability linked to an increase in glutamate signalling is a peculiar trait of the early stages of Alzheimer's disease (AD) and tauopathies, however, a progressive reduction in glutamate release follows in advanced stages. We recently reported that in the

Articles

Extracellular Matrix Proteins and Tools for Cell Culture Optimization

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

细胞外基质蛋白质和细胞培养优化工具

细胞外基质蛋白质，如层粘连蛋白、胶原蛋白和纤连蛋白，可用作细胞培养中的细胞附着基底。

Protocols

Laminin Coating Protocol for Cell Culture

Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.

Human Neural Stem Cells Differentiation with TrueGel3D®

Cultivate ReNcell® human neural stem cells in 3D hydrogels for high-throughput screening using the TrueGel3D® HTS Hydrogel Plate with this protocol.

用于细胞培养的层粘连蛋白包被实验方案

用于细胞培养的层粘连蛋白包被表面需要特定的条件才能获得理想的结果。包含了用于包被盖玻片以进行神经球状体和常规细胞培养的实验方案。

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