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CC12

Sigma-Aldrich

Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6)

liquid, clone DCS-6, Calbiochem®

Synonym(s):

Anti-Bcl-1, Anti-PRAD-1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
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biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

DCS-6, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human, mouse, rat

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

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This Item
SAB4700958OP143OP46
Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6) liquid, clone DCS-6, Calbiochem®

CC12

Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6)

Monoclonal Anti-Cyclin D1 antibody produced in mouse clone DCS-6

SAB4700958

Monoclonal Anti-Cyclin D1 antibody produced in mouse

Anti-MDM2 (Ab-3) Mouse mAb (4B11) liquid, clone 4B11, Calbiochem®

OP143

Anti-MDM2 (Ab-3) Mouse mAb (4B11)

抗-MDM2 (Ab-1) 小鼠mAb (IF2) liquid, clone IF2, Calbiochem®

OP46

抗-MDM2 (Ab-1) 小鼠mAb (IF2)

clone

DCS-6, monoclonal

clone

DCS-6, monoclonal

clone

4B11, monoclonal

clone

IF2, monoclonal

species reactivity

human, mouse, rat

species reactivity

rat, nonhuman primates, human, mouse

species reactivity

mouse, human

species reactivity

human

Gene Information

human ... CCND1(595)
mouse ... Ccnd1(12443)
rat ... Ccnd1(58919)

Gene Information

human ... CCND1(595)

Gene Information

human ... MDM2(4193)
mouse ... Mdm2(17246)

Gene Information

human ... MDM2(4193)
mouse ... Mdm2(17246)

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

purified antibody

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS/1 mouse myeloma cells. Recognizes the cyclin D1 protein.
Recognizes cyclin D1 in MCF-7 and A431 cells. Does not react with cyclins D2 and D3.
This Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6) is validated for use in FC, Frozen Sections, Immunoblotting, IF, IP, Neutralization Studies, Paraffin Sections for the detection of Cyclin D1 (Ab-3).

Immunogen

Human
recombinant human cyclin D1

Application

Flow Cytometry (5 µg/ml; Cat. No. CC12, CC12F)

Frozen Sections (see application references)

Immunoblotting (1 µg/ml)

Immunofluorescence (5 µg/ml; Cat. No. CC12, CC12F)

Immunoprecipitation (see application references)

Neutralization Studies (see application references)

Paraffin Sections (5 µg/ml, heat pre-treatment required)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin.

Analysis Note

Negative Control
trp E (Ab-1)
Positive Control
MCF7, or A431 cells or breast carcinoma tissue

Other Notes

Does not react with cyclins D2 or D3. Staining of paraffin sections can also be detected with pepsin or trypsin pretreatment; light staining will be detected without pretreatment. Do not microwave sections, as this may destroy staining. For immunofluorescence and flow cytometry, we recommend using the FITC conjugate (Cat. No. CC12F). Antibody should be titrated for optimal results in individual systems.
Inaba, T., et al. 1992. Genomics13, 565.
Jiang, W., et al. 1992. Cancer Res.2, 2980.
Pines, J. 1992. Cell Growth Differ.2, 305.
Xiong, Y., et al. 1992. Genomics13, 575.
Xiong, Y., et al. 1992. Cell71, 504.
Motokura, T., et al. 1991. Nature350, 512.
Solomon, M.J., et al. 1990. Cell63, 1013.
Draetta, G., and Beach, D. 1988. Cell54, 17.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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