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Sigma-Aldrich

Anti-α-Tubulin Mouse mAb (DM1A)

liquid, clone DM1A, Calbiochem®

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biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

DM1A, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human, mouse, gerbil, chicken, rat

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... TUBA1A(7846)

General description

Purified mouse monoclonal antibody generated by immunizing Balb/c57 mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~60 kDa tubulin protein.
Recognizes the ~60 kDa tubulin protein found in all eukaryotic cells. May be used as a positive control.
This Anti-α-Tubulin Mouse mAb (DM1A) is validated for use in Immunoblotting, Immunofluorescence for the detection of α-Tubulin.

Immunogen

Chicken
native chick brain microtubules

Application

Immunoblotting (20-100 ng/ml, colorimetric or chemiluminescence; see application references)

Immunofluorescence (5 µg/ml)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin.

Analysis Note

Negative Control
trp E (Ab-1)
Positive Control
Any cell line

Other Notes

Blose, S.H., et al. 1984. J. Cell. Biol.98, 847.
Lin, J.J.C., et al. 1984. In: Monoclonal antibodies and functional cell lines. Kennett, R.H., Bechtol, K.B. and McKearn, T.J., eds. Plenum Publishing Corp.
Lin, J.J.C. 1982. Cold Spring Harbor Symp. on Quant. Biol.46, 769.
Goldman, R.D., et al. 1979. Ann. Rev. Physiol.41, 701.
Goldman, R., Pollard, T. and Rosenbaum, J. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C, Cold Spring Harbor, New York.
Tubulin is found in all cells and is used as a positive control for immunofluorescence or immunoblotting. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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