biological source
mouse
Quality Segment
antibody product type
primary antibodies
clone
4B7, monoclonal
form
liquid
does not contain
preservative
species reactivity
human
should not react with
mouse, rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles, desiccated
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
General description
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the β1 integrin subunit.
Recognizes the β1 integrin subunit in A431 and Jurkat cells.
Immunogen
Human
recombinant, human β1 integrin
Application
Frozen Sections (2 µg/ml)
Immunofluorescence (2.5 µg/ml)
Immunoprecipitation (1 µg/sample)
Paraffin Sections (2 µg/ml, no pre-treatment required)
Immunoblotting (not recommended)
Neutralization Studies (see application references)
Immunofluorescence (2.5 µg/ml)
Immunoprecipitation (1 µg/sample)
Paraffin Sections (2 µg/ml, no pre-treatment required)
Immunoblotting (not recommended)
Neutralization Studies (see application references)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 50 mM sodium phosphate buffer, 50% glycerol.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
A-431 or Jurkat cells or skin tissue
A-431 or Jurkat cells or skin tissue
Other Notes
Akiyama, S.K. 1996. Hum. Cell.9, 181.
Farnoud, M.R. et al. 1996. Int. J. Cancer67, 45.
Morimoto, C. and Tachibana, K. 1996. Hum. Cell.9, 163.
Webb, D.L. et al. 1996. J. Cell. Biochem.61, 127.
Schaller, M.D. et al. 1995. J. Cell. Biol.130, 1181.
Akiyama, S.K. et al. 1994. J. Biol. Chem.269, 15961.
Albelda, S.M. 1993. Lab. Invest.68, 4.
Evans, C.W. 1992. Cell. Biol. Int. Rep.16, 1.
Albelds, S.M. and Buck, C.A. 1990. FASEB J.4, 2868.
Farnoud, M.R. et al. 1996. Int. J. Cancer67, 45.
Morimoto, C. and Tachibana, K. 1996. Hum. Cell.9, 163.
Webb, D.L. et al. 1996. J. Cell. Biochem.61, 127.
Schaller, M.D. et al. 1995. J. Cell. Biol.130, 1181.
Akiyama, S.K. et al. 1994. J. Biol. Chem.269, 15961.
Albelda, S.M. 1993. Lab. Invest.68, 4.
Evans, C.W. 1992. Cell. Biol. Int. Rep.16, 1.
Albelds, S.M. and Buck, C.A. 1990. FASEB J.4, 2868.
Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
10-13 - German Storage Class 10 to 13