Merck
CN
All Photos(1)

Documents

CP28

Sigma-Aldrich

Anti-Fibroblast Antigen Mouse mAb (AS02)

liquid, clone AS02, Calbiochem®

Sign Into View Organizational & Contract Pricing
All Photos(1)
Synonym(s):
Anti-CD90 (Ab-1) (Mouse), Anti-Thy-1 (Ab-1) (Mouse)

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

AS02, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human, monkey

should not react with

rat, mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... FGF22(27006)

General description

Anti-Fibroblast Antigen, mouse monoclonal, clone AS02, detects ~30-31 kDa CD90/Thy-1 antigen in human fibroblasts (non-reducing conditions). It is validated for FC, WB, IF, IP & IHC (frozen sections).
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with X63-Ag.8.653 mouse myeloma cells. Recognizes the ~30-31 kDa fibroblast antigen (CD90/Thy-1) under non-reducing conditions.
Recognizes the ~30-31 kDa CD90/Thy-1 antigen in human fibroblasts under non-reducing conditions.

Immunogen

Human
human fibroblasts

Application

Flow Cytometry (see comments)

Frozen Sections (10 µg/ml, acetone fixation)

Immunoblotting (10 µg/ml, non-reducing only)

Immunofluorescence (10 µg/ml)

Immunoprecipitation (2-10 µg/ml)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 50 mM sodium phosphate buffer, 6% BSA.

Analysis Note

Positive Control
Any fibroblast cell line or skin tissue

Other Notes

Saalbach, A., et al. 1996 J. Invest. Dermatol.106, 1314.
Janin, A., et al. 1990. Clin. Exp. Rheum.8, 237.
Kay, M., et al. 1989. J. Invest. Dermatol.92, 168.
Esterre, P.H., et al. 1984 Cell Mol. Biol.38, 297.
Hoyhtya, M., et al. 1984 Eur. J. Biochem141, 477.
This antibody has been used with magnetic beads to remove fibroblasts from mixed cultures. It is also suitable for flow cytometry studies (see application references). Note that in healthy tissue (skin, placenta, liver, heart), this antibody does not react with endothelial cells. However, endothelial cells activated by PMA, TNFα, or IFNγ may be detected by this antibody, suggesting activated endothelial cells may express CD90 and that CD90 may be an activation marker of human endothelial cells. Antibody should be titrated for optimal results in individual systems.

Legal Information

Manufactured by Dianova, GmbH, Germany.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Deborah Wessels et al.
PloS one, 12(3), e0173400-e0173400 (2017-03-07)
Using unique computer-assisted 3D reconstruction software, it was previously demonstrated that tumorigenic cell lines derived from breast tumors, when seeded in a 3D Matrigel model, grew as clonal aggregates which, after approximately 100 hours, underwent coalescence mediated by specialized cells
T Murakawa et al.
The Journal of thoracic and cardiovascular surgery, 120(4), 712-719 (2000-09-26)
Although cryopreserved tissue allografts are being widely used, long-term degeneration of implanted cryopreserved allografts has become a problem. Although immunologic rejection has been suggested to play a part in this degeneration, cryopreserved allografts are considered to be less immunogenic than
Valentina Hoyos et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 23(9), 1497-1506 (2015-06-19)
Oncolytic adenoviruses (OAdV) represent a promising strategy for cancer therapy. Despite their activity in preclinical models, to date the clinical efficacy remains confined to minor responses after intratumor injection. To overcome these limitations, we developed an alternative approach using the
Katy M Roach et al.
PloS one, 8(12), e85244-e85244 (2014-01-07)
Idiopathic pulmonary fibrosis (IPF) is a common, progressive and invariably lethal interstitial lung disease with no effective therapy. We hypothesised that K(Ca)3.1 K(+) channel-dependent cell processes contribute to IPF pathophysiology. K(Ca)3.1 expression in primary human lung myofibroblasts was examined using
Elena P Moiseeva et al.
PloS one, 8(4), e61579-e61579 (2013-04-27)
Mast cells (MCs) play a central role in the development of many diseases including asthma and pulmonary fibrosis. Interactions of human lung mast cells (HLMCs) with human airway smooth muscle cells (HASMCs) are partially dependent on adhesion mediated by cell
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service