CP45
Anti-Clathrin Heavy Chain Mouse mAb (X22)
liquid, clone X22, Calbiochem®
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About This Item
NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
X22, monoclonal
Species reactivity:
bovine, mouse, rat, human
Application:
—
Citations:
5
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
X22, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
bovine, mouse, rat, human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(Immunoblotting (1:500)
Immunofluorescence (1:1000, indirect)
Immunoprecipitation )
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CLTCL1(8218)
General description
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0-Ag14 mouse myeloma cells. Recognizes the ~180 kDa clathrin heavy chain protein.
Recognizes the ~180 kDa clathrin heavy chain protein.
This Anti-Clathrin Heavy Chain Mouse mAb (X22) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of Clathrin Heavy Chain.
Immunogen
Human
clathrin heavy chain purified from human brain
Application
Immunoblotting (1:500)
Immunofluorescence (1:1000, indirect)
Immunoprecipitation (see comments)
Immunofluorescence (1:1000, indirect)
Immunoprecipitation (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Bauerfeind, R., et al. 1996. J. Neurocytol.25, 701.
Zhang, J.Z., et al. 1994. Cell78, 751.
Herskovits, J.S., et al. 1993. J. Cell Biol.122, 565.
Van Der Bliek, A.M., et al. 1993. J. Cell Biol.122, 553.
Pearse, B.M. and Robinson, M.S. 1990. Ann. Rev. Cell Biol.6, 151.
Brodsky, F.M. 1985. J. Cell Biol.101, 2047.
Zhang, J.Z., et al. 1994. Cell78, 751.
Herskovits, J.S., et al. 1993. J. Cell Biol.122, 565.
Van Der Bliek, A.M., et al. 1993. J. Cell Biol.122, 553.
Pearse, B.M. and Robinson, M.S. 1990. Ann. Rev. Cell Biol.6, 151.
Brodsky, F.M. 1985. J. Cell Biol.101, 2047.
Immunoprecipitation of triskelions allows both the clathrin heavy chain and the associated light chain polypeptides to be examined. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Anna V Salova et al.
Oncotarget, 7(5), 6029-6047 (2015-12-31)
EGF complexed to fluorescent photostable quantum dots by biotin-streptavidin system (bEGF-savQD) is attractive for both the basic research and therapeutic application such as targeted drug delivery in EGF-receptor (EGFR) expressing cancers. However, compared to native EGF, the large size of
Dinah Loerke et al.
Traffic (Copenhagen, Denmark), 6(10), 918-929 (2005-09-06)
The clathrin triskelion is composed of three light chain (LC) and three heavy chain (HC) subunits. Cellular control of clathrin function is thought to be aimed at the LC subunit, mainly on the basis of structural information. To test this
April Thelemann et al.
Molecular & cellular proteomics : MCP, 4(4), 356-376 (2005-01-20)
Overexpression and enhanced activation of the epidermal growth factor (EGF) receptor are frequent events in human cancers that correlate with poor prognosis. Anti-phosphotyrosine and anti-EGFr affinity chromatography, isotope-coded muLC-MS/MS, and immunoblot methods were combined to describe and measure signaling networks
Global Trade Item Number
| SKU | GTIN |
|---|---|
| CP45-100UL | 04055977220995 |