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Merck
CN

CP53

Anti-ICAM-1 (Ab-2) Mouse mAb (W-CAM-1)

liquid, clone W-CAM-1, Calbiochem®

Synonym(s):

Anti-CD54, Anti-Inter-Cellular Adhesion Molecule, Anti-Inter-Cellular Adhesion Molecule, Anti-CD54

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203

Key Documents

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Product Name

Anti-ICAM-1 (Ab-2) Mouse mAb (W-CAM-1), liquid, clone W-CAM-1, Calbiochem®

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

W-CAM-1, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

dilution

(Cell Inhibition Assays
Flow Cytometry
Frozen Sections (1:50-1:100)
Immunoblotting (1:200-1:400)
Immunofluorescence
Immunoprecipitation (10 µL/mg protein lysate)
Paraffin Sections (not recommended))

isotype

IgG2b

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... ICAM1(3383)

Analysis Note

Positive Control
Endothelial cells or tonsil tissue

Application

Cell Inhibition Assays (see comments)

Flow Cytometry (see comments)

Frozen Sections (1:50-1:100)

Immunoblotting (1:200-1:400)

Immunofluorescence (see comments)

Immunoprecipitation (10 µl/mg protein lysate)

Paraffin Sections (not recommended)

Disclaimer

Toxicity: Standard Handling (A)

General description

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing Balb/c splenocytes with p3-NS-1/Ag4-1 (NS1) mouse myeloma cells (see application references). Recognizes the ~85-115 kDa ICAM-1 protein.
Recognizes the ~85-115 kDa ICAM-1 protein in endothelial cells and tonsil tissue.
This Anti-ICAM-1 (Ab-2) Mouse mAb (W-CAM-1) is validated for use in Cell Inhibition Assays, FC, Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of ICAM-1 (Ab-2).

Immunogen

Human
Raji cells

Other Notes

Takami, S., et al. 1998. Circ.97, 721.
Craig, A.G., et al. 1997. Infect. Immun.65, 4580.
DeGraba, T.J. 1997. Neurology49, S15.
Elangbam, C.S., et al. 1997. Vet. Pathol. 34, 61.
McCormick, C.J., et al. 1997. J. Clin. Invest.15, 2521.
Acevedo, A., et al. 1993. Am. J. Pathol.143, 774.
De Fougerolles, A.R., et al. 1993. J. Exp. Med.177, 1187.
Fawcett, J., et al. 1992. Nature360, 481.
Vazeux, R. et al. 1992. Nature360, 485.
Boyd, A.W., et al. 1989. Blood73, 1896.
This antibody will block aggregation of cell lines mediated by ICAM-1 and blocks homotypic binding of purified populations of activated T- and B-lymphocytes and aggregation of mixed T- and B-cell blasts. Will inhibit T-cell adhesion to normal human endothelial cells. Activation induced by cell-cell contact (mixed lymphocyte reaction, T cell mediated B-cell activation) is significantly inhibited. This antibody will also block elements of both effector arms of the immune system (cytotoxic cell function and Ig production). This antibody has been reported to work for immunofluorescence and flow cytometry studies. Antibody should be titrated for optimal results in individual systems.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 10 mM PBS, 0.2% BSA, pH 7.4.

Preparation Note

Following initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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