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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
2E2-D3, monoclonal
Species reactivity:
human
Application:
—
Citations:
-
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
2E2-D3, monoclonal
form
liquid
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(Immunoblotting (1-5 µg/mL)
Immunocytochemistry (10 µg/mL)
Paraffin Sections (5 µg/mL))
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... TARDBP(23435)
General description
Purified mouse monoclonal antibody. Recognizes the ~45 kDa TARDBP protein.
Recognizes the ~45 kDa TARDBP protein in A431 and HeLa cells and human leiomyosarcoma tissue.
This Anti-TARDBP Mouse mAb (2E2-D3) is validated for use in Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of TARDBP.
Immunogen
Full-length, recombinant human TARDBP (aa 1-261), expressed as a GST fusion protein.
Application



Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (5 µg/ml)
Physical form
In PBS, pH 7.7.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Negative Control
293T cells
293T cells
Positive Control
A431 cells, Human leiomyosarcoma tissue, HeLa cells
A431 cells, Human leiomyosarcoma tissue, HeLa cells
Other Notes
Ilieva, E,V., et al. 2010. Free Radic. Biol. Med.48, 1302.
Wils H, et al. 2010. Proc. Natl. Acad. Sci. U S A107 3858.
Foulds, P,G., et al. 2009. Acta Neuropathol.118 647.
Wils H, et al. 2010. Proc. Natl. Acad. Sci. U S A107 3858.
Foulds, P,G., et al. 2009. Acta Neuropathol.118 647.
Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)
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Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| DR1075-100UG | 04055977226058 |