E5014
Benzonase ® Salt Tolerant Endonuclease
≥200 U/uL, for nucleic acid removal at high salt concentration
Synonym(s):
Endonuclease, nuclease
biological source
Escherichia coli (recombinant, expressed in E.coli)
Quality Level
form
buffered aqueous glycerol solution
mol wt
calculated mol wt 27 kDa
concentration
≥200 units/μL
storage temp.
-10 to -25°C
General description
The best-in-class solution for nucleic acid removal at high salt concentration. The Benzonase® Salt Tolerant endonuclease is an enzyme, different in amino acids sequence from other Benzonase® endonuclease references. It was designed with state-of-the-art protein engineering capabilities, to ensure the highest activity at high salt concentrations. The protein is a monomer with a molecular weight of about 27 kDa . The protein has an isoelectric point (pI) at pH 9.68. It is functional between pH 6 and 10, and from 0 °C to above 37 °C. Mg2+ (1–2 mM) is required for enzyme activity.
Application
Benzonase® Salt Tolerant endonuclease degrades both DNA and RNA, whether single stranded, double-stranded, linear, circular or supercoiled. No base preference is observed. As with all endonucleases, Benzonase® Salt Tolerant endonuclease hydrolyzes internal phosphodiester bonds present between the nucleotides. Upon complete digestion, all free nucleic acids present in solution are reduced to 5’-monophosphate terminated oligonucleotides, which are three to five bases in length.
Features and Benefits
- Active at higher salt concentration, up to1M NaCl
- Non-animal origin, recombinant from E.Coli, High Purity
- No post-translational modifications
- High batch to batch reproducibility
- Free of detectable protease activity
- Does not contain any antimicrobial preservatives or protein stabilizers except glycerol
- Compatible with Deviron® detergents for cell lysis
Preparation Note
-10°C to -25°C
Legal Information
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
DEVIRON is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Regulatory Information
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