ECM210
QCM Endothelial Cell Invasion Assay (24 well, colorimetric)
This QCM Endothelial Cell Invasion Assay provides an in vitro model to quickly screen factors that can regulate endothelial invasion. The assay is performed in an invasion chamber using a basement membrane protein coated on the porous insert.
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About This Item
Quality Level
manufacturer/tradename
Chemicon®
QCM
technique(s)
cell based assay: suitable
detection method
colorimetric
shipped in
wet ice
General description
Introduction
Endothelial cells (EC) invade through the basement membrane (BM) to form sprouting vessels. The invasion process consists of the secretion of matrix metalloproteases (MMP) to degrade basement membrane, the activation of endothelial cells, and the migration of EC across the basement membrane. The understanding of EC invasion is important for studying the mechanism of angiogenesis in injured tissue as well as in disease such as cancer.
Cell migration may be evaluated through several different methods, the most widely accepted of which is the Boyden Chamber assay. The Boyden Chamber system uses two-chamber system which a porous membrane provides an interface between two chambers. Cells are seeded in the upper chamber and chemoattractants placed in the lower chamber. Cells in the upper chamber migrate toward the chemoattractants by passing through the porous membrane to the lower chamber. Migratory cells are then stained and quantified.
Application
Apoptosis & Cancer
Cell Structure
Packaging
Preparation Note
Other Notes
2. Cell Stain Solution: (Part No. 90144)* One bottle.
3. Extraction Buffer: (Part No. 90145) One bottle.
4. 24-well Stain Extraction Plate: (Part No. 2005871) One each.
4. 24-well Stain Extraction Plate: (Part No. 2005871) One each.
5. 96-well Stain Quantitation Plate: (Part No. 2005870) One each.
6. Cotton Swabs: (Part No. 10202) Fifty each.
7. Forceps: (Part No. 10203) One each.
Legal Information
Disclaimer
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2
Storage Class Code
3 - Flammable liquids
Flash Point(F)
53.6 °F
Flash Point(C)
12 °C
Certificates of Analysis (COA)
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Articles
Cell based angiogenesis assays to analyze new blood vessel formation for applications of cancer research, tissue regeneration and vascular biology.
在癌症研究、组织再生和血管生物学应用中通过以细胞为基础的血管生成检测来分析新血管的形成。
Related Content
Cell migration is stimulated and directed by interaction of cells with the extracellular matrix (ECM), neighboring cells, or chemoattractants. Cell migration participates in morphogenic processes, wound healing and tumor metastasis. Specifically, inhibiting tumor invasion by blocking tumor cell chemotaxis has been a major focus of research. Tumor cell invasion, marked by degradation of ECM, is also directly correlated with metastatic potential.
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