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ECM555

QCM ECMatrix Cell Invasion Assay, 96-well (8 µm), fluorimetric

The CHEMICON Cell Invasion Assay Kit employs a 96-well plate, and provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model.

Synonym(s):

8 μm pore cell invasion assay

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.32
eCl@ss:
32161000
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Quality Level

100

species reactivity

human

manufacturer/tradename

Chemicon®, QCM

technique(s)

activity assay: suitable, cell based assay: suitable

detection method

fluorometric

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

QCM ECMatrix Cell Invasion Assay involves invaded cells at the bottom of the insert membrane and can be obtained by treating the membrane with cell detachment buffer. The cells are lysed and detected by the patented CyQuant GR dye. This dye is a green-fluorescent dye and binds well with cellular nucleic acids to emit a strong fluorescence. This assay kit provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model.

Application

QCM ECMatrix Cell Invasion Assay, 96-well (8 μm), fluorimetric has been used in the cell invasion assay of:

  • human cervical carcinoma
  • osteosarcoma
  • breast cancer cells
  • colorectal cancer cells
Ideal for screening pharmacological agents, evaluating MMPs or proteases responsible for cell invasion, and gene function analysis in transfected cells

Features and Benefits

  • The assay does not require cell labeling, scraping, washing, or counting
  • Suitable for large-scale screening and quantitative comparison of multiple samples due to homogenous fluorescence detection and 96 well insert
  • Enables a quick and efficient system for the quantitative determination of several factors for cell invasion

Other Notes

Sterile 96-well Cell Invasion Plate Assembly: (Part No. 90200) One 96-well feeder tray, and one 96-well Cell Invasion Chamber plate with ECMatrixTM -coated inserts.

96-well Cell Culture Tray: (Part No. 90129) One 96-well feeder tray.

Cell Detachment Solution: (Part No. 90131) One bottle - 16 mL.

4X Cell Lysis Buffer: (Part No. 90130) One bottle - 16 mL.

CyQuant GR Dye: (Part No. 90132) One vial - 75 μL

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

CorrosionEnvironment
GHS05,GHS09

signalword

Danger

Storage Class

10 - Combustible liquids

wgk

WGK 2

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1

Certificates of Analysis (COA)

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Targeting of Integrin {beta}1 and Kinesin 2{alpha} by MicroRNA 183.
Li G, Luna C, Qiu J, Epstein DL, Gonzalez P
The Journal of Biological Chemistry null
Increases in mitochondrial biogenesis impair carcinogenesis at multiple levels.
Xiao Wang,Carlos T Moraes
Molecular Oncology null
Sodium arsenite inhibits migration of extravillous trophoblast cells in vitro.
Chi-Shan Li,Rita Loch-Caruso
Reproductive Toxicology null
Sheeba Irshad et al.
Cancer research, 77(5), 1083-1096 (2017-01-14)
Cancer cells tend to metastasize first to tumor-draining lymph nodes, but the mechanisms mediating cancer cell invasion into the lymphatic vasculature remain little understood. Here, we show that in the human breast tumor microenvironment (TME), the presence of increased numbers
Adam S Adler et al.
Nature genetics, 38(4), 421-430 (2006-03-07)
Gene expression signatures encompassing dozens to hundreds of genes have been associated with many important parameters of cancer, but mechanisms of their control are largely unknown. Here we present a method based on genetic linkage that can prospectively identify functional
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Global Trade Item Number

SKUGTIN
ECM55504053252745188

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