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ECM555

Sigma-Aldrich

QCM ECMatrix Cell Invasion Assay, 96-well (8 µm), fluorimetric

The CHEMICON Cell Invasion Assay Kit employs a 96-well plate, and provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model.

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Synonym(s):
8 μm pore cell invasion assay
eCl@ss:
32161000

Quality Level

100

species reactivity

human

manufacturer/tradename

Chemicon®
QCM

technique(s)

activity assay: suitable
cell based assay: suitable

detection method

fluorometric

shipped in

wet ice

Related Categories

General description

QCM ECMatrix Cell Invasion Assay involves invaded cells at the bottom of the insert membrane and can be obtained by treating the membrane with cell detachment buffer. The cells are lysed and detected by the patented CyQuant GR dye. This dye is a green-fluorescent dye and binds well with cellular nucleic acids to emit a strong fluorescence. This assay kit provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model.

Application

QCM ECMatrix Cell Invasion Assay, 96-well (8 μm), fluorimetric has been used in the cell invasion assay of:

  • human cervical carcinoma
  • osteosarcoma
  • breast cancer cells
  • colorectal cancer cells
Ideal for screening pharmacological agents, evaluating MMPs or proteases responsible for cell invasion, and gene function analysis in transfected cells

Features and Benefits

  • The assay does not require cell labeling, scraping, washing, or counting
  • Suitable for large-scale screening and quantitative comparison of multiple samples due to homogenous fluorescence detection and 96 well insert
  • Enables a quick and efficient system for the quantitative determination of several factors for cell invasion

Components

Sterile 96-well Cell Invasion Plate Assembly: (Part No. 90200) One 96-well feeder tray, and one 96-well Cell Invasion Chamber plate with ECMatrixTM -coated inserts.

96-well Cell Culture Tray: (Part No. 90129) One 96-well feeder tray.

Cell Detachment Solution: (Part No. 90131) One bottle - 16 mL.

4X Cell Lysis Buffer: (Part No. 90130) One bottle - 16 mL.

CyQuant GR Dye: (Part No. 90132) One vial - 75 μL

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

CorrosionEnvironment
GHS05,GHS09

Signal Word

Danger

Hazard Statements

H318 - H410

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

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Sheeba Irshad et al.
Cancer research, 77(5), 1083-1096 (2017-01-14)
Cancer cells tend to metastasize first to tumor-draining lymph nodes, but the mechanisms mediating cancer cell invasion into the lymphatic vasculature remain little understood. Here, we show that in the human breast tumor microenvironment (TME), the presence of increased numbers
Sodium arsenite inhibits migration of extravillous trophoblast cells in vitro.
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Andreas M Stark,Bartosz Anuszkiewicz,Rolf Mentlein,Toshiyuki Yoneda,H Maximilian Mehdorn et al.
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Targeting of Integrin {beta}1 and Kinesin 2{alpha} by MicroRNA 183.
Li G, Luna C, Qiu J, Epstein DL, Gonzalez P
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