PAI Activity Assay

Research Category
Cell Structure

The PAI Activity Assay Kit provides a quick, efficient & sensitive system for evaluation of inhibition of uPA activity by PAI & for screening of uPA inhibitors.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

The CHEMICON PAI Activity Assay Kit provides a quick, efficient and sensitive system for evaluation of inhibition of uPA activity by PAI and for screening of uPA inhibitors. The assay is colorimetric and does not require radioactivity or fluorescence equipment. The assay is sensitive over an inhibition range of 0.05-50 units of uPA activity.

The CHEMICON PAI Activity Assay Kit utilizes a chromogenic substrate, which is cleaved by active uPA, detectable by its Optical Density at 405nm (OD405). Addition of PAI sample blocks the cleavage of substrate by uPA.

The CHEMICON PAI Assay Kit is ideal for measurement of PAI inhibitory activity toward uPA in purified preparations and cell culture, as well as in serum where pathological conditions such as sepsis exist. The assay is also useful for screening inhibitors of uPA.

Each CHEMICON PAI Activity Assay Kit contains sufficient reagents for the evaluation of 96 samples, including uPA standard from human urine and E.Coli-expressed recombinant protein corresponding to amino acids 24 to 427 of human PAI-1 (mature form) as positive control. PAI-1 positive control contains a mixture of the active and latent forms, with >45% of active form. Duplicate or triplicate samples are suggested.

The CHEMICON PAI Activity Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.

Test Principle:

The CHEMICON PAI Activity Assay Kit utilizes a chromogenic substrate, which is cleaved by active uPA, detectable by its Optical Density at 405nm (OD405). Addition of PAI sample blocks the cleavage of substrate by uPA.

Application:

The CHEMICON PAI Assay Kit is ideal for measurement of PAI inhibitory activity toward uPA in purified preparations and cell culture, as well as in serum where pathological conditions such as sepsis exist. The assay is also useful for screening inhibitors of uPA.

Each CHEMICON PAI Activity Assay Kit contains sufficient reagents for the evaluation of 96 samples, including uPA standard from human urine and E. coli expressed recombinant protein corresponding to amino acids 24 to 427 of human PAI-1 (mature form) as positive control. PAI-1 positive control contains a mixture of the active and latent forms, with >45% of active form. Duplicate or triplicate samples are suggested.

The CHEMICON PAI Activity Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.

uPA Standard: (Part No. 90092) One lyophilized vial, 1000 units, of uPA from human urine.

Chromogenic Substrate: (Part No. 90545) One 5 mg bottle of Tripeptide with pNA group.

Assay Buffer, 10X: (Part No. 90091) One 5 mL bottle.

PAI-1 Positive Control: (Part No. 90093) One 400 μL vial containing 20 μg of purified PAI-1 with 0.1% BSA.

96 wells

Store kit materials at -20°C for up to 12 months. Store reconstituted chromogenic substrate at 2-8°C.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany