ECM625
IN VITRO ANGIOGENESIS ASSAY KIT
Synonym(s):
Angiogenesis assay kit
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About This Item
species reactivity
vertebrates
Quality Level
packaging
pkg of 1 kit
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
shipped in
dry ice
storage temp.
2-8°C
General description
The CHEMICON in vitro angiogenesis assay kit provides a convenient system for evaluation of tube formation by endothelial cells in a convenient 96-well format. When cultured on ECMatrix, a solid gel of basement proteins prepared from the Engelbreth Holm-Swarm (EHS) mouse tumor, these endothelial cells rapidly align and form hollow tube-like structures. Tube formation is a multi-step process involving cell adhesion, migration, differentiation, and growth.
ECMatrix is optimized for maximal tube-formation. For assaying inhibitors or stimulators of tube formation, simply premix the endothelial cell suspension with different concentrations of the inhibitor or stimulator to be tested, before adding the cells to the top of the ECMatrix.
Application
- human umbilical vein cells (HUVEC)
- primary human retinal microvascular cells
- platelet derived growth factor receptor-β (PDGFRβ)/PAE cells
- bovine capillary endothelial (BCE) cells
Preparation Note
Other Notes
- ECMatrixTM Gel Solution: (Part No. 90060) Five 1 mL bottles.
- ECMatrixTM Diluent Buffer, 10X: (Part No. 90061) One 1 mL bottle.
Legal Information
Disclaimer
Storage Class Code
12 - Non Combustible Liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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