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ESG1106

Sigma-Aldrich

ESGRO® Recombinant Mouse LIF Protein

ESGRO Leukemia Inhibitory Factor (LIF) supplement for mouse ES cell culture. Each vial contains 10^6 units/ml

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Synonym(s):
Mouse LIF, Mouse Leukemia Inhibitory Factor, Murine LIF, Murine LIF, Mouse LIF, mLIF, mLIF
eCl@ss:
32160801
NACRES:
NA.75

Quality Level

100

Assay

>95% (active component mLIF, SDS-PAGE)

form

liquid

manufacturer/tradename

Chemicon®

concentration

1000000 units/mL

technique(s)

cell culture | stem cell: suitable

input

sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

NCBI accession no.

NM_008501

UniProt accession no.

P09056

shipped in

wet ice

Gene Information

mouse ... LIF(16878)

Related Categories

General description

Mouse Leukemia Inhibitory Factor (LIF, mLIF) promotes self-renewal and long-term maintenance of pluripotent mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells by suppressing spontaneous differentiation.
The ESGRO mouse LIF medium supplement is supplied in active units/mL for consistent performance from lot to lot. Each lot of ESGRO supplement undergoes stringent quality control testing to ensure reliable inhibition of mouse pluripotent stem cell differentiation. ESGRO mouse LIF supplement may be used for feeder based and feeder free culture of mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells.

Application

Applications for ESGRO Supplement include use as a reagent for the in vitro maintenance of the pluripotential phenotype of murine ES cells.

SUGGESTED PROTOCOLS:

ES Cells: In D3 and MBL-1 pluripotential ES cell cultures it is routinely found that 1000 U of ESGRO Supplement per 1.0 mL of tissue culture media is required to maintain ES cells with a stem cell phenotype. Similar concentrations of mLIF have also been used for germline transmission of genetically altered ES cells (E3).

At the recommended concentration 107 units of ESGRO Supplement is sufficient for 10.0 L of tissue culture media and 106 units of ESGRO Supplement is sufficient for 1.0 L of tissue culture media.
ESGRO Leukemia Inhibitory Factor (LIF) supplement for mouse ES cell culture. Each vial contains 10^6 units/ml

Physical form

ESGRO Supplement is supplied in liquid form as 106 Units in 1.0 mL of phosphate buffered saline with 1% w/v bovine serum albumin BSA as a carrier for stability.
Format: Purified

Preparation Note

ESGRO® supplied 0.22 micron sterile filtered, and tested negative in mycoplasma tests.

Storage and Stability

ESGRO Supplement is shipped at ambient temperature. A cold pack may be included for customer relations, however it is truly not needed. Extensive stability tests have been performed with ESGRO Supplement during development. This product is stable for at least 18 months from the date of manufacture, in the concentrated form or diluted in sterile tissue culture media, with no loss of activity on ES cells. For long term storage it is recommended that ESGRO Supplement be stored at 4°C. Freeze-thawing will reduce potency. It is recommended that prior to use, ESGRO Supplement should be diluted in sterile tissue culture media and aliquoted to a convenient concentration, then stored at 4°C. Freeze-thawing should be avoided. Product is stable for a minimum of 7 days at 37°C, 5% CO2 incubator during the culture of ES cells.

Analysis Note

Specific Activity: ESGRO® Supplement is assessed both on mouse embryonic stem cells (D3 & MBL-1) and on murine M1 myeloid leukemic cells(4). A standard of 50 Units is defined as the concentration of ESGRO Supplement in 1.0mL of tissue culture medium that induces the differentiation of 50% of M1 colonies(4). 106 units is equivalent to approximately 10 μg of pure protein, and is sufficient to treat 1L of ES cell culture media.

Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/mL Murine myeloid leukemic, M1 Assay: Specific Activity >/= 108 units/mg

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
ESGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Ashley L Fritz et al.
Biotechnology and bioengineering, 112(1), 209-219 (2014-07-30)
The induction of pluripotency from adult cells has enormous potential in regenerative medicine. While initial efforts to study mechanisms and improve efficiency of induced pluripotent stem cell (iPSC) reprogramming focused on the direct roles of transcriptional regulators, increasing evidence indicates
Systematic delineation of optimal cytokine concentrations to expand hematopoietic stem/progenitor cells in co-culture with mesenchymal stem cells.
Andrade, Pedro Z, et al.
Molecular Biosystems, 6, 1207-1215 (2010)
Kelsey H Elliott et al.
eLife, 9 (2020-10-03)
Despite a common understanding that Gli TFs are utilized to convey a Hh morphogen gradient, genetic analyses suggest craniofacial development does not completely fit this paradigm. Using the mouse model (Mus musculus), we demonstrated that rather than being driven by
Role of Tet proteins in 5mC to 5hmC conversion, ES-cell self-renewal and inner cell mass specification.
Ito S. et al.
Nature null
Short RNAs are transcribed from repressed polycomb target genes and interact with polycomb repressive complex-2.
Kanhere, Aditi, et al.
Molecular Cell, 38, 675-688 (2010)
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