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EZBRAIN42

Sigma-Aldrich

Human Amyloid β42 Brain ELISA

This Human Amyloid β42 Brain ELISA is used to measure & quantify Amyloid β42 levels in Neuroscience research.

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eCl@ss:
32161000

Quality Level

200

species reactivity

human

assay range

sensitivity: 8 pg/mL
(lowest level of Amyloid β1-42 standard; 50 μL sample size)
standard curve range: 16-500 pg/mL

technique(s)

ELISA: suitable

detection method

colorimetric

Gene Information

human ... APP(351)

Related Categories

General description

Amyloid beta peptides have been implicated in the etiology of Alzheimer’s disease. Amyloid beta 40 is the most prominent peptide and Amyloid beta 42 is the neurotoxic form. The Amyloid beta 42/40-ratio (AB ratio) has been reported as a better indicator of the Alzheimer pathology. Millipore’s Human Amyloid β42 Brain ELISA kit is used for the measurement of Amyloid beta 42 in brain samples and for other tissue samples in a 96-well format. This assay is for research use only and appropriate for the in vitro detection of human Amyloid β42 peptides in brain samples from e.g. Guinea pig, transgenic hAmyloid mice and cell extracts.

Specificity

The Amyloid β42 ELISA uses monoclonal anti-Aβ antibodies with high selectivity for human Aβ. The capture antibody recognizes the C-terminal end of Amyloid β1-42, which causes a high selectivity for Aβ42. The cross-reactivity of the used antibodies to other Amyloid peptides was tested by ELISA and BIACORE and shows no significant cross-reactivity to Aβ1-38, Aβ1-39, Aβ1-42, Aβ1-43 and Aβ1-44.

Application

Research Category
Neuroscience
Research Sub Category
Alzheimer′s Disease
This Human Amyloid β42 Brain ELISA is used to measure & quantify Amyloid β42 levels in Neuroscience research.
This assay requires 50 µl of sample and is an overnight assay.
Used to detect/quantify: Amyloid β42

Storage and Stability

Components in the kit can be stored up to 2 weeks at 2-8°C

Other Notes

Please contact Technical Service for linearity of dilution.

Disclaimer

For research use only. Not for use in diagnostic procedures.

Pictograms

CorrosionExclamation mark
GHS05,GHS07

Signal Word

Warning

Hazard Statements

H290 - H317 - H412

Hazard Classifications

Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Regulatory Information

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Jesse E Hanson et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(24), 8277-8288 (2014-06-13)
Extensive evidence implicates GluN2B-containing NMDA receptors (GluN2B-NMDARs) in excitotoxic-insult-induced neurodegeneration and amyloid β (Aβ)-induced synaptic dysfunction. Therefore, inhibiting GluN2B-NMDARs would appear to be a potential therapeutic strategy to provide neuroprotection and improve cognitive function in Alzheimer's disease (AD). However, there
Michael B McFerrin et al.
Annals of clinical and translational neurology, 4(7), 466-477 (2017-07-12)
The highly conserved 14-3-3 proteins interact with key players involved in Parkinson's disease (PD) and other neurodegenerative disorders. We recently demonstrated that 14-3-3 phosphorylation is increased in PD models and that increased 14-3-3 phosphorylation reduces the neuroprotective effects of 14-3-3
André E S Simões et al.
BMC genomics, 14, 181-181 (2013-03-19)
Simultaneous isolation of nucleic acids and proteins from a single biological sample facilitates meaningful data interpretation and reduces time, cost and sampling errors. This is particularly relevant for rare human and animal specimens, often scarce, and/or irreplaceable. TRIzol(®) and TRIzol(®)LS
Y Goll et al.
Neuro-degenerative diseases, 13(2-3), 58-60 (2013-11-07)
Most Alzheimer's disease (AD) cases arise sporadically and may involve innate immune activation of microglial expressed Toll-like receptors regulated through the myeloid differentiation protein 88 (MyD88) pathway. It was the aim of this study to test the innate immune involvement
Elliott J Mufson et al.
Journal of neuropathology and experimental neurology, 71(11), 1018-1029 (2012-10-26)
Hippocampal precursor of nerve growth factor (proNGF)/NGF signaling occurs in conjunction with β-amyloid (Aβ) accumulations in Alzheimer disease (AD). To assess the involvement of this pathway in AD progression, we quantified these proteins and their downstream pathway activators in postmortem
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