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EZBRAIN-SET

Sigma-Aldrich

Human Amyloid β40 and Amyloid β42 Brain ELISA

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species reactivity

human

Quality Level

200

assay range

sensitivity: 6.0 pg/mL
(The lowest level of Amyloid β1-40 standard used in this assay ; 50 μL sample size)
sensitivity: 8 pg/mL
(The lowest level of Amyloid ß1-42 standard used in this assay; 50 μL sample size)
inter-assay cv: <10
intra-assay cv: <10
standard curve range: 16-500 pg/mL

technique(s)

ELISA: suitable

detection method

colorimetric

storage temp.

2-8°C

Gene Information

human ... APP(351)

Specificity

The Amyloid β40 ELISA uses monoclonal anti-Aβ antibodies with high selectivity for human Aβ. The capture antibody recognizes the C-terminal end of Amyloid β1-40, which causes a high selectivity for Aβ 40. The cross-reactivity of the used antibodies to other Amyloid peptides was tested by ELISA and BIACORE and shows no significant cross-reactivity to Aβ1-38, Aβ1-39, Aβ1-42, Aβ1-43 and Aβ1-44. The Amyloid β42 ELISA uses monoclonal anti-Aβ antibodies with high selectivity for human Aβ. The capture antibody recognizes the C-terminal end of Amyloid β1-42, which causes a high selectivity for Aβ42. The cross-reactivity of the used antibodies to other Amyloid peptides was tested by ELISA and BIACORE and shows no significant cross-reactivity to Aβ1-38, Aβ1-39, Aβ1-42, Aβ1-43 and Aβ1-44.


Used to detect/quantify: Amyloid β40 & Amyloid β42

Application

These assays require 50 µl of sample and are overnight assays.

Packaging

96-well plate

Storage and Stability

Components in the kit can be stored up to 2 weeks at 2-8°C

Other Notes

Contact technical support for information

Disclaimer

For research use only. Not for use in diagnostic procedures.

Regulatory Information

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