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Merck
CN

HATF00010

Immobilon® -NC Nitrocellulose Membrane

1 roll, 33 cm x 3 m, 0.45 µm pore size, Triton-free, nitrocellulose-based transfer membrane

Synonym(s):

Western blotting membrane, blotting membrane, nitrocellulose transfer membrane, transfer membrane

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About This Item

UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22
Material:
mixed cellulose esters (MCE) membrane, nitrocellulose membrane, plain filter, white filter
Detection method:
chemiluminescent, colorimetric, fluorometric, radioactive
Pore size:
0.45 μm pore size
Compatibility:
for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with India ink, for use with Ponceau-S red

Key Documents

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Product Name

Immobilon®-NC Transfer Membrane, 1 roll, 33 cm x 3 m, 0.45 µm pore size, Triton-free, mixed cellulose esters transfer membrane

material

mixed cellulose esters (MCE) membrane, nitrocellulose membrane, plain filter, white filter

feature

hydrophilic

manufacturer/tradename

Immobilon®

technique(s)

western blot: suitable

filter L × W

33 cm × 3 m

pore size

0.45 μm pore size

capacity

117 μg/cm2 adsorption capacity (insulin), 160 μg/cm2 adsorption capacity (BSA), 259 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with India ink, for use with Ponceau-S red

detection method

chemiluminescent, colorimetric, fluorometric, radioactive

shipped in

ambient

Quality Level

400

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General description

Immobilon-NC (HATF) membrane is composed of a mixed cellulose esters matrix. It is devoid of surfactants that may interfere with cell wall integrity during cell growth. This membrane is recommended for colony and plaque lifts.

Application

Immobilon-NC Transfer Membrane has been used in western blot analysis.

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Flame
GHS02

signalword

Danger

hcodes

H228

Hazard Classifications

Flam. Sol. 1

Storage Class

4.1B - Flammable solid hazardous materials

wgk

WGK 3

Certificates of Analysis (COA)

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Bing Wang et al.
Oncology letters, 13(5), 3494-3500 (2017-05-23)
The aim of the present study was to gain insight into the molecular mechanism of gefitinib resistance in non-small cell lung cancer (NSCLC), and demonstrate whether long noncoding RNA (lncRNA) expression signatures differ between gefitinib-sensitive PC9 and gefitinib-resistant PC9 (PC9-R)
Kanika Verma et al.
Nature communications, 11(1), 2926-2926 (2020-06-12)
Metabolic changes alter the cellular milieu; can this also change intracellular protein folding? Since proteostasis can modulate mutational buffering, if change in metabolism has the ability to change protein folding, arguably, it should also alter mutational buffering. Here we find
1H-Indole-3-Carbonyl-Thiazole-4-Carboxylic Acid Methyl Ester Blocked Human Glioma Cell Invasion via Aryl Hydrocarbon Receptor?s Regulation of Cytoskeletal Contraction
Zhao L, et al.
BioMed Research International (2020)
Yunhua Hou et al.
Experimental cell research, 387(2), 111750-111750 (2019-12-02)
Lymphoma, a malignant tumor, is mainly characterized by painless lymph node enlargement and hepatosplenomegaly. At present, lymphoma is mainly treated by radiation, chemical drugs, bone marrow transplantation and surgery. However, due to the high degree of heterogeneity, lymphomas are highly
Yanfang Guo et al.
Minerva endocrinologica, 45(1), 29-35 (2018-12-12)
Vitamin D, a fat-soluble secosteroid, plays a key role in several metabolic diseases like diabetes. Diabetes is becoming a third leading chronic disease in the world, which seriously threatens human health. In the current study, we found the beneficial effects
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Protocols

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

WB检测样本前处理：细胞裂解和蛋白提取

Western blotting前用于培养组织和细胞的细胞裂解和有效蛋白提取的样品制备实验方案。

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