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HATF00010

Immobilon® -NC Nitrocellulose Membrane

1 roll, 33 cm x 3 m, 0.45 µm pore size, Triton-free, nitrocellulose-based transfer membrane

Synonym(s):

Western blotting membrane, blotting membrane, nitrocellulose transfer membrane, transfer membrane

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About This Item

UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22
Material:
mixed cellulose esters (MCE) membrane, nitrocellulose membrane, plain filter, white filter
Detection method:
chemiluminescent, colorimetric, fluorometric, radioactive
Pore size:
0.45 μm pore size
Compatibility:
for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with India ink, for use with Ponceau-S red
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Product Name

Immobilon®-NC Transfer Membrane, 1 roll, 33 cm x 3 m, 0.45 µm pore size, Triton-free, mixed cellulose esters transfer membrane

Quality Level

400

material

mixed cellulose esters (MCE) membrane, nitrocellulose membrane, plain filter, white filter

feature

hydrophilic

manufacturer/tradename

Immobilon®

technique(s)

western blot: suitable

filter L × W

33 cm × 3 m

pore size

0.45 μm pore size

capacity

117 μg/cm2 adsorption capacity (insulin), 160 μg/cm2 adsorption capacity (BSA), 259 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with India ink, for use with Ponceau-S red

detection method

chemiluminescent, colorimetric, fluorometric, radioactive

shipped in

ambient

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General description

Immobilon-NC (HATF) membrane is composed of a mixed cellulose esters matrix. It is devoid of surfactants that may interfere with cell wall integrity during cell growth. This membrane is recommended for colony and plaque lifts.

Application

Immobilon-NC Transfer Membrane has been used in western blot analysis.

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Flame
GHS02

signalword

Danger

hcodes

H228

Hazard Classifications

Flam. Sol. 1

Storage Class

4.1B - Flammable solid hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yunhua Hou et al.
Experimental cell research, 387(2), 111750-111750 (2019-12-02)
Lymphoma, a malignant tumor, is mainly characterized by painless lymph node enlargement and hepatosplenomegaly. At present, lymphoma is mainly treated by radiation, chemical drugs, bone marrow transplantation and surgery. However, due to the high degree of heterogeneity, lymphomas are highly
Bing Wang et al.
Oncology letters, 13(5), 3494-3500 (2017-05-23)
The aim of the present study was to gain insight into the molecular mechanism of gefitinib resistance in non-small cell lung cancer (NSCLC), and demonstrate whether long noncoding RNA (lncRNA) expression signatures differ between gefitinib-sensitive PC9 and gefitinib-resistant PC9 (PC9-R)
Hong Wen et al.
Reproductive sciences (Thousand Oaks, Calif.), 27(6), 1357-1364 (2020-02-15)
Selective intrauterine growth restriction (sIUGR) is a disorder of monochorionic (MC) twin pregnancies. However, the underlying mechanism remains largely unknown. Trophoblast cells are the major component of the placenta. Dysfunction of trophoblast cells is associated with placental dysfunction. Our previous
Kuanxiang Sun et al.
BioMed research international, 2020, 7342817-7342817 (2020-07-01)
Phosphatidylserine flippase (P4-ATPase) transports PS from the outer to the inner leaflet of the lipid bilayer in the membrane to maintain PS asymmetry, which is important for biological activities of the cell. ATP11A is expressed in multiple tissues and plays
Lin Liu et al.
OncoTargets and therapy, 12, 11507-11516 (2020-01-11)
ARHGAP10 belongs to the ARHGAP family, which is downregulated in certain human tumors. However, the detailed function of ARHGAP10 remains unclear in human colon carcinoma (CRC). In the current study, we aimed to explore the role of ARHGAP10 in the
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Protocols

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

WB检测样本前处理：细胞裂解和蛋白提取

Western blotting前用于培养组织和细胞的细胞裂解和有效蛋白提取的样品制备实验方案。

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Western Blotting Tools

Western blotting is one of the most commonly used techniques in the lab, yet difficulties persist in obtaining consistent, quality results. We’ve been helping scientists publish their Western blots for decades, with continued innovation and steadfast technical support. Explore our expanded portfolio of products, including optimized reagents for chemiluminescent and Ḁuorescent Westerns, as well as the SNAP i.d.® system, which reduces blocking, washing and antibody incubation time from hours to minutes.

Global Trade Item Number

SKUGTIN
HATF0001004053252672811

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