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HCYP4MAG-64K

MILLIPLEX® Human Cytokine/Chemokine Panel 4

Configurable Human Cytokine/Chemokine 20-Plex Panel 4

Synonym(s):

Human cytokine multiplex kit, Luminex® human cytokine immunoassay, Millipore human cytokine panel

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.47
eCl@ss:
32161000

Product Name

MILLIPLEX® Human Cytokine/Chemokine Panel 4,

description

Configurable Human Cytokine/Chemokine 20-Plex Panel 4

Quality Segment

200

species reactivity

human

packaging

pkg of 1 ea

manufacturer/tradename

Milliplex®

assay range

accuracy: 101%
(BAFF/Blys), accuracy: 101%
(BRAK/CSCL14), accuracy: 101%
(IL-28B), accuracy: 102%
(MPIF-1/CL23), accuracy: 106%
(IL-19), accuracy: 110%
(HCC-4/CCL16), accuracy: 110%
(IL-34), accuracy: 89%
(IL-32α), accuracy: 95%
(IL-35), accuracy: 99%
(IL-37), sensitivity: 0.30 ng/mL
(YKL40/CHI3L1), sensitivity: 0.8 ng/mL
(IL-24), sensitivity: 0.8 ng/mL
(IL-35), sensitivity: 0.9 ng/mL
(BAFF/BLyS), sensitivity: 10.5 ng/mL
(HMGB1/HMG1), sensitivity: 100.8 pg/mL
(CCL28), sensitivity: 121.5 pg/mL
(IL-34), sensitivity: 17.2 pg/mL
(IL-28B/IFN-λ3), sensitivity: 248.2 pg/mL, sensitivity: 27.7 pg/mL
(IL-14/α-Taxilin), sensitivity: 28.4 pg/mL
(HCC-4), sensitivity: 3.5 pg/mL
(IL-36β), sensitivity: 32.1 pg/mL
(IL-32α), sensitivity: 33.2 pg/mL
(IL-38), sensitivity: 375.6 pg/mL
(IFNβ), sensitivity: 4.7 pg/mL
(CXCL16), sensitivity: 5.2 pg/mL
(IL-37/IL-1F7), sensitivity: 6.6 pg/mL
(MIP-4/PARC), sensitivity: 607 pg/mL
(BRAK), sensitivity: 7.8 pg/mL
(MPIF-1), standard curve range: 0.1-100 ng/mL
(YKL40/CHI3L1), standard curve range: 0.44-400 ng/mL
(BAFF/Blys), standard curve range: 0.7-750 ng/mL
(IL-24), standard curve range: 0.8-800 ng/mL
(IL-35), standard curve range: 10-10,000 pg/mL
(MPIF-1/CL23), standard curve range: 2-2,000 pg/mL
(IL-36β), standard curve range: 2.0-2,000 ng/mL
(HMGB1), standard curve range: 24-25,000 pg/mL
(IL-14), standard curve range: 244-250,000 pg/mL
(IL-19), standard curve range: 29-30,000 pg/mL
(IL-28B), standard curve range: 3-3,000 pg/mL
(CXCL16), standard curve range: 39-40,000 pg/mL
(IL-32α), standard curve range: 488-500,000 pg/mL
(BRAK/CXCL14), standard curve range: 488-500,000 pg/mL
(IFNβ), standard curve range: 49-50,000 pg/mL
(IL-38), standard curve range: 5-5,000 pg/mL
(IL-37), standard curve range: 5-5,000 pg/mL
(MIP-4/PARC/CCL18), standard curve range: 98-100,000 pg/mL
(CCL28), standard curve range: 98-100,000 pg/mL
(IL-34)

technique(s)

multiplexing: suitable

input

cell culture supernatant(s)
serum
plasma

detection method

fluorometric (Luminex® xMAP® technology)

shipped in

wet ice

storage temp.

2-8°C

General description

“Cytokine” is a general term used for a diverse group of soluble proteins and peptides which act as regulators under both normal and pathological conditions to modulate the functional activities of individual cells and tissues. These proteins also mediate direct interactions between cells and regulate processes taking place in the extracellular environment. The cytokine group of proteins includes lymphokines, interferons, colony stimulating factors and chemokines. Cytokine and chemokine research plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, as well as disease states such as inflammatory disease, allergic reactions, irritable bowel disease (IBD), sepsis, and cancer.

Application

MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze up to 20 analytes in human serum, plasma, and cell culture supernatants.

Analytes included: BAFF/Blys, BRAK/CXCL14, CCL28, CXCL16, HCC-4/CCL16, HMGB1, IFNβ, IL-14, IL-19, IL-24, IL-28B, IL-32α, IL-34, IL-35, IL-36β/IL-1F8, IL-37/IL-1F7, IL-38/IL-1F10, MIP-4, MPIF/CCL23, YKL40

Note: HMGB1 can be measured in serum and plasma samples only.

Assay Characteristics: Refer to kit protocol for assay cross-reactivity, sensitivity, precision, and accuracy.

Features and Benefits

  • Emerging Biomarker Coverage: Quantify 20 understudied targets like IL-37 (anti-inflammatory), YKL-40 (fibrosis marker), and HMGB1 (DAMP molecule), unlocking insights into novel immune-regulatory pathways and disease mechanisms.
  • Precision Dilution Protocols: Optimized 1:4 dilution for serum/plasma balances sensitivity and matrix interference, while tissue culture compatibility supports in vitro model validation.
  • Modular Assay Design: Curate analyte subsets (e.g., CCL28 for mucosal immunity, IL-28B for viral response) to align with niche hypotheses, maximizing resource efficiency.
  • Dual Incubation Flexibility: Choose overnight (16–18 hr) for low-abundance targets (e.g., IL-34) or fast 2-hour workflows for high-concentration analytes.
  • Niche Pathway Profiling: Targets include IL-36β (psoriasis), BRAK/CXCL14 (tumor suppression), and IL-35 (autoimmunity), bridging gaps in inflammation and oncology research.

Legal Information

Luminex is a registered trademark of Luminex Corporation
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

For research use only. Not for use in diagnostic procedures.

Label License/Sticker for Assay Product:

By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

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signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

target_organs

Respiratory Tract

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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