usage
kit sufficient for 96 wells
species reactivity
human
technique(s)
multiplexing: suitable
detection method
fluorometric (Luminex® xMAP®)
storage temp.
2-8°C
General description
Cytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses.“Cytokine” is a general term used for a diverse group of soluble proteins and peptides which act as regulators under both normal and pathological conditions to modulate the functional activities of individual cells and tissues. These proteins also mediate direct interactions between cells and regulate processes taking place in the extracellular environment. Cytokines differ from hormones in that they act on a wider spectrum of target cells. The cytokine group of proteins includes lymphokines, interferons, colony stimulating factors and chemokines. Growth factors are involved in the stimulation of target cell survival, proliferation, differentiation with effects on angiogenesis, vasculogenesis, cell migration, apoptosis, wound healing and embryogenesis. Cytokine, chemokine and growth factor research plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process, and disease states such as infectious disease, osteoarthritis, respiratory disease, IBD, sepsis, allergic reactions as well as neurologic, metabolic and cardiovascular disease and even cancer.
MILLIPLEX® offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust.
Each MILLIPLEX® panel and kit includes:
MILLIPLEX® offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust.
Each MILLIPLEX® panel and kit includes:
- Quality controls (QCs) to qualify assay performance
- Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency
- Detection antibody cocktails designed to yield consistent analyte profiles within panel In addition each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility.
Application
Simultaneously analyze up to 48 multiple cytokine, chemokine, and growth factor biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in cell culture samples.
Analytes Availables:
CD40L, EGF, Eotaxin, FGF-2, Flt-3 ligand, Fractalkine, G-CSF, GM-CSF, GROα, IFNα2, IFNγ, IL-1α, IL-1β, IL-1ra, IL-2, , IL-3IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IL-17E/IL-25, IL-17F, IL-18, IL-22, IL-27, IP-10, MCP-1, MCP-3, M-CSF, MDC (CCL22), MIG, MIP-1α, MIP-1β, PDGF-AA, PDGF-AB/BB, RANTES, TGFα, TNFα, TNFβ, VEGF-A
Application Notes:
This is an overnight or two-hour incubation assay.
This assay requires 25 µL of cell culture supernatant per well.
This kit may be used for the analysis of any or all of the Human Cytokine/Chemokine/Growth Factor Panel A analytes in human tissue/cell lysate and culture supernatant samples.
Cross Reactivity: There was no or negligible cross-reactivity observed between the antibodies for any single analyte and any of the other analytes in this panel, except IL-12 (p40). IL-12 (p40) exhibited a cross-reactivity of approximately 8% with IL-12 (p70). This is not unexpected since the p40 subunit is a constituent of IL-12 (p70).
Analytes Availables:
CD40L, EGF, Eotaxin, FGF-2, Flt-3 ligand, Fractalkine, G-CSF, GM-CSF, GROα, IFNα2, IFNγ, IL-1α, IL-1β, IL-1ra, IL-2, , IL-3IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IL-17E/IL-25, IL-17F, IL-18, IL-22, IL-27, IP-10, MCP-1, MCP-3, M-CSF, MDC (CCL22), MIG, MIP-1α, MIP-1β, PDGF-AA, PDGF-AB/BB, RANTES, TGFα, TNFα, TNFβ, VEGF-A
Application Notes:
This is an overnight or two-hour incubation assay.
This assay requires 25 µL of cell culture supernatant per well.
This kit may be used for the analysis of any or all of the Human Cytokine/Chemokine/Growth Factor Panel A analytes in human tissue/cell lysate and culture supernatant samples.
Cross Reactivity: There was no or negligible cross-reactivity observed between the antibodies for any single analyte and any of the other analytes in this panel, except IL-12 (p40). IL-12 (p40) exhibited a cross-reactivity of approximately 8% with IL-12 (p70). This is not unexpected since the p40 subunit is a constituent of IL-12 (p70).
Legal Information
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
Target Organs
Respiratory Tract
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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