HEXSM-170K

MILLIPLEX^® Human Exosome Characterization Panel

Name: MILLIPLEX<SUP>®</SUP> Human Exosome Characterization Panel
Brand: MM

Configurable Human Exosome Characterization 9-Plex Panel

Synonym(s):

EV multiplex kit, Human exosome multiplex kit, Luminex EV panel, Luminex human exosome immunoassay, Millipore human exosome panel, extracellular vesicle multiplex kit

About This Item

NACRES:

NA.55

UNSPSC Code:

12161503

Key Documents

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Quality Level

200

species reactivity

human

packaging

pkg of 1 ea

manufacturer/tradename

Milliplex^®

parameter

25 μL sample volume (per well)

assay range

intra-assay cv: ≤20%, standard curve range: 0.4-1,500 ng/mL
(CD9), standard curve range: 0.5-2,000 ng/mL
(CD81), standard curve range: 0.6-2,500 ng/mL
(Flotillin-1), standard curve range: 0.9-3,500 ng/mL
(Syntenin-1), standard curve range: 122-500,000 pg/mL
(CD63), standard curve range: 24-100,000 pg/mL
(Calreticulin, GAPDH), standard curve range: 49-200,000 pg/mL
(TSG101), inter-assay cv: ≤10%

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex^® xMAP^® technology)

shipped in

wet ice

storage temp.

2-8°C

Related Categories

Immunoassay Platform Solutions

MILLIPLEX^® Multiplex Assays for Luminex^® Instruments

General description

Cells release extracellular vesicles (EV), including exosomes, as part of normal physiological processes including removal of waste and regulation of intercellular communication. Furthermore, increased exosome release has been implicated in the progression of various disease states and other abnormalities such as cancer and neurodegeneration. Exosome identification, classification, and differentiation from other extracellular vesicles is often complicated due to the variability in isolation and preparation protocols, exosome biogenesis, as well as exosome source. Commonly associated exosome-enriched markers include tetraspanins CD9, CD63, and CD81, cargo sorting-associated markers TSG101 and Flotillin-1, and biogenesis marker syntenin-1. Other markers, including Ago2, GAPDH, and Calreticulin, may or may not be found in exosome-enriched samples, but this can vary depending on source, isolation method, and/or EV biogenesis mechanism.

Application

MILLIPLEX^® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze up to 9 analytes in exosomes isolated from serum, plasma, and tissue culture supernatants.

Analytes included: Argonaute-2 (Ago2), Calreticulin (CALR), CD9, CD63, CD81, Flotillin-1 (FLOT-1), GAPDH, Syntenin-1 (Syn-1), and TSG101

Note: The Ago2 bead is static and will always be included in the kit format.

Assay Characteristics: Refer to kit protocol for assay cross-reactivity, sensitivity, precision, and accuracy.

Features and Benefits

Comprehensive Marker Coverage: This panel includes a range of markers associated with exosome biogenesis, cargo sorting, and general ceullar function. This allows for detailed characterization of exosomes, providing insights into their biological roles and implications in various diseases.
Quantitative and Qualitative Analysis: This dual capability allows researchers to assess both the presence and concentration of specific exosomal markers providing a comprehesive understanding of exosomal profiles.
Flexibility in Exosomal Isolation Methods: This kit allows users to employ various exosome isolation methods according to their laboratory preferences and established protocols.
Configurable Kit: Design your multiplex kit by selecting from a variety of available analytes, tailoring assays to meet your specific research needs.
Control Included: The MCF7:IGF-1 Cell Lysate is included as a control for the qualitative assay of Argonaute-2. This control ensures the reliability of the qualitative results, helping to verify experimental conditions and support data interpretation.
Flexibility in Lysis Buffer Selection: Researchers can use their preferred lysis buffer, with recommendations provided for Cell Signaling Lysis Buffer.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Disclaimer

For research use only. Not for use in diagnostic procedures.

Label License/Sticker for Assay Product:

By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

pictograms

GHS08,GHS05,GHS07,GHS09

signalword

Danger

target_organs

Respiratory Tract

Storage Class

10 - Combustible liquids

wgk

WGK 3

hcodes

H302 + H312 + H332,H315,H317,H318,H373,H410,EUH430

pcodes

P201 - P202 - P273 - P280 - P305 + P351 + P338 - P391 - P405 - P501

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2

Regulatory Information

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MILLIPLEX^® Human Exosome Characterization Panel