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HMYOMAG-56K

Millipore

MILLIPLEX® Human Myokine Magnetic Bead Panel

The Human Myokine Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 15 myokine protein biomarkers in human serum, plasma and cell/tissue culture samples.

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eCl@ss:
32161000

Quality Level

200

species reactivity

human

manufacturer/tradename

Milliplex®

assay range

accuracy: 100%
(Apelin)
accuracy: 92%
(IL-15)
accuracy: 96%
(FSTL-1)
accuracy: 97%
(LIF)
accuracy: 98%
(Oncostatin M (OSM))
sensitivity: 0.9 pg/mL
(MinDC+2SD; IL-6)
sensitivity: 14 pg/mL
(MinDC+2SD; FGF-21)
sensitivity: 259 pg/mL
(MinDC+2SD; Myostatin/GDF8)
sensitivity: 281 pg/mL
(MinDC+2SD; Irisin)
sensitivity: 4 pg/mL
(MinDC+2SD; LIF)
sensitivity: 40 pg/mL
(MinDC+2SD; Fractalkine/CX3CL1)
sensitivity: 5 pg/mL
(MinDC+2SD; IL-15)
sensitivity: 59 pg/mL
(MinDC+2SD; Osteocrin/Musclin)
sensitivity: 6 pg/mL
(MinDC+2SD; Oncostatin M (OSM))
sensitivity: 64 pg/mL
(MinDC+2SD; Apelin)
sensitivity: 7.5 ng/mL
(MinDC+2SD; Osteonectin (SPARC))
sensitivity: 737 pg/mL
(MinDC+2SD; Follistatin-like Protein 1 (FSTL1))
sensitivity: 815 pg/mL
(MinDC+2SD; Erythropoietin (EPO))
sensitivity: 9 pg/mL
(MinDC+2SD; BDNF)
sensitivity: 9.8 pg/mL
(MinDC+2SD; FABP3)
standard curve range: 0.6-2,400 pg/mL
(IL-6)
standard curve range: 10-40,000 pg/mL
(LIF)
standard curve range: 122-500,000 pg/mL
(Apelin)
standard curve range: 2-10,000 pg/mL
(BDNF)
standard curve range: 2-10,000 pg/mL
(IL-15)
standard curve range: 2-6,000 pg/mL
(Oncostatin M (OSM))
standard curve range: 24-100,000 pg/mL
(FABP3)
standard curve range: 244-1,000,000 pg/mL
(Erythropoietin (EPO))
standard curve range: 244-1,000,000 pg/mL
(Irisin)
standard curve range: 488-2,000,000 pg/mL
(Follistatin-like Protein 1 (FSTL1))
standard curve range: 488-2,000,000 pg/mL
(Myostatin/GDF8)
standard curve range: 49-200,000 pg/mL
(Fractalkine/CX3CL1)
standard curve range: 49-200,000 pg/mL
(Osteocrin/Musclin)
standard curve range: 5-20,000 pg/mL
(FGF-21)
standard curve range: 7-30,000 ng/mL
(Osteonectin (SPARC))
inter-assay cv: <15%
intra-assay cv: <10%
(Apelin)
inter-assay cv: <15%
intra-assay cv: <10%
(BDNF)
inter-assay cv: <15%
intra-assay cv: <10%
(Erythropoietin (EPO))
inter-assay cv: <15%
intra-assay cv: <10%
(FABP3)
inter-assay cv: <15%
intra-assay cv: <10%
(FGF-21)
inter-assay cv: <15%
intra-assay cv: <10%
(Follistatin-like Protein 1 (FSTL1))
inter-assay cv: <15%
intra-assay cv: <10%
(Fractalkine/CX3CL1)
inter-assay cv: <15%
intra-assay cv: <10%
(IL-15)
inter-assay cv: <15%
intra-assay cv: <10%
(IL-6)
inter-assay cv: <15%
intra-assay cv: <10%
(Irisin)
inter-assay cv: <15%
intra-assay cv: <10%
(LIF)
inter-assay cv: <15%
intra-assay cv: <10%
(Myostatin/GDF8)
inter-assay cv: <15%
intra-assay cv: <10%
(Oncostatin M (OSM))
inter-assay cv: <15%
intra-assay cv: <10%
(Osteocrin/Musclin)
inter-assay cv: <20%
intra-assay cv: <10%
(Osteonectin (SPARC))

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

Related Categories

General description

Muscle contraction during physical activity is an important activator of the release of the myokines. Myokines not only act in an autocrine and/or paracrine manner in regulation of skeletal muscle growth, but they also act as endocrine hormones to mediate inter-organ crosstalk. The myokine theory provides molecular mechanistic explanations for exercise-induced metabolic changes in liver and adipose tissue and for the profound changes found in immune and neuronal systems.

MILLIPLEX® Human Myokine Panel is a15-plex kit to be used for the simultaneous quantification of any or all of the following analytes in human serum,plasma and cell/tissue supernatant samples: Fractalkine/CX3CL1, BDNF, Oncostatin M (OSM), Osteonectin (SPARC), LIF, FGF-21, Erythropoietin (EPO), IL-15, IL-6, Apelin, Myostatin/GDF8, Irisin, Osteocrin/Musclin, FABP3 and Follistatin-Like Protein (FSTL 1). This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Metabolism

Application

  • Analytes: Apelin (APLN), Brain-derived Neurotrophic Factor (BDNF), Erythropoeitin (EPO), Fatty Acid-Binding Protein 3 (FABP3), Fibroblast Growth Factor 21 (FGF21), Fractalkine (CX3CL1), Follistatin-Like 1 Protein (FSTL-1), IL-6, IL-15, Irisin, Leukemia Inhibitory Factor (LIF), Myostatin (MSTN/GDF8), Oncostatin M (OSM), Osteocrin (OSTN/Musclin), Osteonectin (SPARC)
  • Recommended Sample Type: Human serum, plasma, and cell/tissue culture supernatants or lysates
  • NOTE: Substantial amounts of BDNF are stored in circulating platelets and subsequently released upon platelet activation. Therefore, platelet-poor plasma is critical to ensure accurate measurement of circulating levels of BDNF.
  • Recommended Sample Dilution: 25 μL per well of 1:2 diluted serum or plasma. Cell/tissue culture samples may be run neat or appropriately diluted in control media.
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C or 2 hours at room temperature (20-25°C)
  • Research Category: Bone
  • Research Subcategory: Metabolism

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Other Notes

Please contact Technical Service for linearity of dilution.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Regulatory Information

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Xi He et al.
Signal transduction and targeted therapy, 6(1), 427-427 (2021-12-18)
Abnormal glucose and lipid metabolism in COVID-19 patients were recently reported with unclear mechanism. In this study, we retrospectively investigated a cohort of COVID-19 patients without pre-existing metabolic-related diseases, and found new-onset insulin resistance, hyperglycemia, and decreased HDL-C in these
Ayla Karine Fortunato et al.
Journal of immunology research, 2018, 9675216-9675216 (2018-07-27)
Strength exercise is a strategy applied in sports and physical training processes. It may induce skeletal muscle hypertrophy. The hypertrophy is dependent on the eccentric muscle actions and on the inflammatory response. Here, we evaluate the physiological, immunological, and inflammatory
Kelly Ann Hutchinson et al.
Frontiers in physiology, 10, 1188-1188 (2019-10-28)
It is recommended that women accumulate 150-min of weekly moderate-intensity physical activity (MPA) when pregnant. Engaging in regular physical activity (PA) confers many health benefits to both the mother and the fetus. However, the molecular mechanisms by which these health
Joanna D C C Lima et al.
Journal of cachexia, sarcopenia and muscle, 10(5), 1045-1059 (2019-07-06)
Cachexia is a paraneoplastic syndrome related with poor prognosis. The tumour micro-environment contributes to systemic inflammation and increased oxidative stress as well as to fibrosis. The aim of the present study was to characterise the inflammatory circulating factors and tumour
Javier Diaz-Castro et al.
Frontiers in nutrition, 8, 761213-761213 (2021-11-09)
Practicing exercise is one of the best strategies to promote well-being and quality of life, however physical activity in schoolchildren and adolescents is developed in an unpredictable, intermittent way and in short periods. There are relatively few intervention studies investigating

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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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