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IM57

Anti-MT1-MMP (Ab-4) Mouse mAb (113-5B7)

liquid, clone 113-5B7, Calbiochem®

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About This Item

NACRES:
NA.43
UNSPSC Code:
12352203

Key Documents

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Product Name

Anti-MT1-MMP (Ab-4) Mouse mAb (113-5B7), liquid, clone 113-5B7, Calbiochem®

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

113-5B7, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human, rabbit, rat, mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

dilution

(Immunoblotting (10 µg/mL)
Immunofluorescence
Immunoprecipitation
Paraffin Sections )

isotype

IgG3

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... MMP14(4323)

Analysis Note

Positive Control
HT-1080 or ConA-treated MD-MB-231 cells

Application


Immunoblotting (10 g/ml)
Immunofluorescence (see application references)
Immunoprecipitation (see application references)
Paraffin Sections (see application references)

Disclaimer

Toxicity: Standard Handling (A)

General description

Purified mouse monoclonal antibody. Recognizes the ~60 kDa MTI-MMP protein in overexpressing cells.
Recognizes the ~60 kDa MTI-MMP protein in HT-1080 cells and in ConA-treated MD-MB-231 cells.
This Anti-MT1-MMP (Ab-4) Mouse mAb (113-5B7) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation, Paraffin Sections for the detection of MT1-MMP (Ab-4).

Immunogen

Human
a synthetic peptide (CDGNFDTVAMLRGEM) corresponding to amino acids 319-333 in the hemopexin-like domain of human MT1-MMP

Legal Information

Manufactured by Daiichi Fine Chemicals C0., Ltd., Not available for sale in Japan.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Sakakibara, M., et al. 1999. Cancer85, 231-239.
Mattei, M.G., et al. 1997. Genomics:40, 168.
Okada, A., et al. 1997. J. Cell. Biol.147, 67.
Okumura, Y., et al. 1997. FEBS Lett.402, 181.
Ueno, H., et al. 1997. Cancer Res.57, 2055.
Strongin, A.Y., et al. 1995. J. Biol. Chem.270 5331.
Takino, T., et al. 1995. J. Biol. Chem.270, 23013.
Cottam, D. W. and Rees, R. C., 1993. Intl J. Oncol.2, 861.
Stetler-Stevenson, W. G., et al. 1993. FASEB J.7, 1434.
Fridman, R., et al. 1992. J. Biol. Chem.267, 15389.
Woessner, J. F., 1991. FASEB J.5, 2145.
Liotta, L. A. and Stetler-Stevenson, W. G., 1990. in Sem. Cancer Biol., ed. M. M. Gottesman. Vol. 1(2), 99.
Goldberg, G.I., et al. 1989. PNAS86, 8207.
This antibody cross-reacts slightly with human MMP-3. To induce MT1-MMP in MD-MB-231 cells, treat with 1-5 µg/ml ConA for 24-48 h. Antibody should be titrated for optimal results in individual systems.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Stephanie W Watts et al.
American journal of physiology. Heart and circulatory physiology, 292(5), H2438-H2448 (2007-01-24)
Arterial remodeling occurs in response to mechanical and neurohumoral stimuli. We hypothesized that veins, which are not exposed to higher pressures in hypertension, would demonstrate less active remodeling than arteries. We assessed remodeling with two standard measures of arterial remodeling:
Yong-Feng Yang et al.
Scientific reports, 6, 30505-30505 (2016-07-29)
The trabecular meshwork (TM) tissue controls drainage of aqueous humor from the anterior chamber of the eye primarily by regulating extracellular matrix (ECM) remodeling by matrix metalloproteinases (MMPs). Glaucomatous TM tissue is stiffer than age-matched controls, which may be due
Ricardo Cruz-Acuña et al.
Journal of cell science, 132(20) (2019-09-29)
Synthetic hydrogels with controlled physicochemical matrix properties serve as powerful in vitro tools to dissect cell-extracellular matrix (ECM) interactions that regulate epithelial morphogenesis in 3D microenvironments. In addition, these fully defined matrices overcome the lot-to-lot variability of naturally derived materials

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