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Merck
CN

IPFL07810

Immobilon®-FL PVDF Membrane

1 roll, 7 x 8.4cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

Synonym(s):

Western blotting membrane, blotting membrane, transfer membrane

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About This Item

UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22

Key Documents

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Product Name

Immobilon®-FL PVDF Transfer Membrane, 0.45 μm pore size, 7 X 8.4 cm sheets

material

PVDF membrane
plain filter
white filter

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable
western blot: suitable

filter L × W

7 cm × 8.4 cm

pore size

0.45 μm pore size

capacity

155 μg/cm2 adsorption capacity (insulin)
 205 μg/cm2 adsorption capacity (BSA)
 300 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black
for use with CPTS
for use with Coomassie brilliant blue
for use with Ponceau-S red

detection method

chemiluminescent
colorimetric
fluorometric

shipped in

ambient

Quality Level

400

Related Categories

Application

Immobilon®-FL Transfer Membrane has been used in western blotting.

Features and Benefits

Immobilon®-FL Transfer Membrane:
  • Designed for optimal fluorescent immunodetection application
  • Extremely low background improves the sensitivity of all fluorescence based immunodetection
  • Compatible with all commonly used fluorescent probes across a wide range of excitation/emission wavelengths
  • For standard chemiluminescent or chromogenic detection

General description

The Immobilon®-FL transfer membrane is a polyvinylidene fluoride (PVDF) microporous membrane for binding proteins transferred from a variety of gel matrices. The Immobilon®-FL membrane has a nominal pore size of 0.45 μm and is recommended for blotting proteins with molecular weights greater than 20 kilodaltons (kDa). The membrane exhibits very low autofluorescence across a wide range of excitation/emission wavelengths. This property makes it ideal for blotting applications involving fluorescence-based immunodetection.
Filter Code: IPFL

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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