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ISEQ00010

Millipore

Immobilon®-PSQ PVDF Membrane

1 roll, 27 cm x 3.75 m, 0.2 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

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Synonym(s):
Western blotting membrane, blotting membrane, transfer membrane
eCl@ss:
32031602

material

PVDF membrane
plain filter
white filter

Quality Level

400

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable
western blot: suitable

filter L × W

27 cm × 3.75 m

pore size

0.2 μm pore size

capacity

262 μg/cm2 adsorption capacity (insulin)
 340 μg/cm2 adsorption capacity (BSA)
 448 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black
for use with CPTS
for use with Colloidal gold
for use with Coomassie brilliant blue
for use with India ink
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
for use with Toluidine blue
for use with Transillumination

detection method

chemiluminescent
colorimetric
radioactive

shipped in

ambient

Related Categories

General description

The Immobilon®-PSQ membrane is a 0.2 μm microporous membrane made up of polyvinylidene fluoride (PVDF). It has been developed to maximize protein binding. Immobilon®-PSQ membrane is recommended for direct protein sequencing and immunodetection following electroblotting of protein from electrophoresis gels (or dot binding of purified protein samples), especially for proteins with molecular weights less than 20,000. This membrane is compatible with tank and semi-dry electroblotting systems.

Application

Immobilon®-PSQ PVDF Membrane has been used in western blot.

Features and Benefits

Immobilon®-PSQ Membrane:
  • 0.2 μm pore size and a large internal structure
  • Higher protein adsorption and sequencing yields than other membranes
  • Prevents blow-through of low molecular weight proteins

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Protocols

Stripping and Reprobing Western Blotting Membranes

This page shows and discusses three protocols for stripping and reprobing a western blot membrane.

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

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