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LP02

Sigma-Aldrich

Anti-LDL Receptor Mouse mAb (15C8)

liquid, clone 15C8, Calbiochem®

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biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

15C8, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human, bovine

should not react with

rabbit

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

isotype

IgG2a

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... LDLR(3949)

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0-Ag14 myeloma cells (see application references). Recognizes the ~160 kDa LDL Receptor protein.
Recognizes the ~160 kDa LDL receptor protein in HT1080 and HepG2 cells.
This Anti-LDL Receptor Mouse mAb (15C8) is validated for use in ELISA, Flow Cytometry, Frozen Sections, Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of LDL Receptor.

Immunogen

Bovine
Epitope: first cysteine-rich ligand binding domain
a trypic digest of bovine low density lipoprotein receptor

Application


ELISA (0.5-1 g/ml)
Flow Cytometry (see application references)
Frozen Sections (1-5 g/ml)
Immunoblotting (1-5 g/ml, see application references)
Immunocytochemistry (1-5 g/ml, IF)
Immunoprecipitation (1-2 g/sample)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 0.05 M sodium phosphate buffer, 0.2% gelatin.

Analysis Note

Positive Control
Liver sections, HT1080 cells, HepG2 cells

Other Notes

Will displace 50-70% of LDL binding to the receptor. For immunoblotting, include 2 mM Ca+2 with the antibody and avoid EDTA. This antibody has been reported to bind best to the unreduced LDL-R (see specificity application reference). Antibody should be titrated for optimal results in individual systems.
van Driel, I.R., et al. 1987. J. Biol. Chem.262, 16127.
van Driel, I.R., et al. 1987. J. Biol. Chem.262, 17443.
Russell, D.W., et al. 1984. Cell37, 577.
Achneider, W.J., et al. 1983. J. Cell Biol.97, 1635.
Cummings, R.D., et al. 1983. J. Biol. Chem.258, 15261.
Schneider, W.J., et al. 1982 J. Biol. Chem.257, 2664.
Goldstein, J.L., et al. 1979. Nature279, 679.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Kei Horihata et al.
Reproduction, fertility, and development, 29(7), 1280-1286 (2016-05-18)
The corpus luteum (CL) synthesises and secretes progesterone (P4), which is essential for the establishment and maintenance of pregnancy in mammals. P4 is synthesised from cholesterol. Cholesterol is internalised by low-density lipoprotein receptor (LDLR) and/or scavenger receptor B1 (SR-BI), and

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