LSKMAGG
PureProteome Protein G Magnetic Bead System
The PureProteome Protein G Magnetic Bead System is a powerful system that helps researchers purify proteins by maximizing recovery and eliminating variability
Synonym(s):
Protein G Magnetic Beads, PureProteome Magnetic Beads
Select a Size
About This Item
packaging
pkg of 2 × 1 mL
manufacturer/tradename
PureProteome
technique(s)
depletion: suitable (serum), immunoprecipitation (IP): suitable, protein purification: suitable
particle size
10 μm
capacity
2.5-3.5 μg/μL, suspension binding capacity (rabbit IgG)
shipped in
wet ice
General description
Application
- serum depletion
- immunoprecipitation
- protein purification
- for isolation of the immunoglobulin G (IgG) fraction from a small volume of plasma
- for co-immunoprecipitation (Co-IP)
Features and Benefits
- PureProteome™ Magnetic Beads are very well suited for fully automated purification process using the KingFisher Duo particle processor, it is not dependent on the cell lysate clarification step
- The entire process is reproducible, and the results are comparable to that of a standard protocol using the PureProteome Magnetic Stand
- PureProteome Magnetic Beads is compatible with any automated system for low, medium, or high-throughput sample preparation.
Legal Information
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Articles
PureProteome™ Protein A and G Magnetic beads provide a rapid and reproducible means to purify immunoglobulins (IgG) using the KingFisher Duo particle processor.
通过 PureProteome™ Protein A 和 Protein G 磁珠，可利用 KingFisher Duo 磁珠纯化仪快速、可重现地纯化免疫球蛋白 (IgG)。
Related Content
Purification of recombinant proteins expressed in E.coli requires many time-consuming steps. To liberate the protein of interest, traditional bacterial lysis relies on the addition of lysozyme and a combination of sonication and repeated freeze/thaw cycles to break the bacterial cell wall. Disruption of the cell is accompanied by an increase in the viscosity of the suspension, due to the release of DNA. An endonuclease is added to digest the DNA, thus reducing the viscosity of the lysate. Finally, to render the lysate compatible with traditional purification methods, insoluble cell debris must be removed by centrifugation.
Western blotting is one of the most commonly used techniques in the lab, yet difficulties persist in obtaining consistent, quality results. We’ve been helping scientists publish their Western blots for decades, with continued innovation and steadfast technical support. Explore our expanded portfolio of products, including optimized reagents for chemiluminescent and Ḁuorescent Westerns, as well as the SNAP i.d.® system, which reduces blocking, washing and antibody incubation time from hours to minutes.
Immunoprecipitation (IP) is a powerful technique for proteomic screening, biomarker discovery, and signaling network elucidation. It is frequently used to enrich target proteins from complex samples such as cell lysates or extracts. Traditional IP protocols use Protein A, Protein G or a mixture of Protein A and G coupled to a solid support resin, such as agarose beads, to capture an antigen/antibody complex in solution. As the number of samples increase, the traditional, manual IP method can be time-consuming. Processing of multiple IP reactions in parallel can introduce complexity, variability and pipetting errors, which may affect reproducibility.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| LSKMAGG10 | 04053252323188 |
| LSKMAGG02 | 04053252373053 |