CellASIC ONIX gradient plate for mammalian cells (4 chamber)

Convert your microscope to a live cell imaging system – the CellASIC® ONIX2 system allows precise manipulation of the cell culture environment for dynamic live cell imaging and analysis using mammalian, yeast, and bacterial cells.

We demonstrate here the ability to conduct gene expression analysis using the CellASIC™ ONIX System with M04S microfluidic plates. Ultimately, directly correlating gene expression patterns to phenotypes observed during live cell imaging can provide powerful, meaningful functional genomics data, revealing signaling networks and novel biomolecular interactions.

We used the CellASIC™ ONIX MicroFluidic System, in conjunction with the M04S microfluidic plate, to achieve perfusion-based microenvironment control and study drug-induced cell death of 3D cultures of MCF7 cells in Matrigel®. The microfluidic chamber recreates the physiologic mass transport condition for optimized cell health. Four upstream fluidic channels allow controlled exposure of the cells to different solutions. The plate can also be cultured in a standard incubator using a dedicated gravity-driven flow channel. The cells are in contact with a #1.5 thickness (170 μm) optical glass surface, enabling high quality imaging using an inverted microscope. An integrated micro-incubator system delivers temperature and gas control to the microfluidic chambers.