By Western blot the antibody recognizes the 83 kDa light chain of human Factor VIII. No cross reactivity with von Willebrand factor.

Epitope: 83 kDa light chain

Purified Human Factor VIII.

Anti-Factor VIII Antibody, 83 kDa light chain is an antibody against Factor VIII for use in ELISA & WB.

ELISA (indirect): 1:16,000.

Western blotting: Factor VIII makes up only about 1% of the ciruculating FactorVIII/vWF complex with the bulk of the complex being vWF (99% by mass, see Blood 58:1-13, 1981). Thus it is recommended that the FactorVII/vWF be precipitated out of plasma prior to detection of Factor VII with MAB038. Also, when blotting plasma preparations, background, non-specific bands, caused by the serum proteins are common. More complex blocking solutions or higher salt concentrations can help remove non-specific bands. Rotblat, et al (1985) Biochemistry 24(16):4294-4300. Should be consulted for additional tips with regard to detecting Factor VII via westerns.



Optimal working dilutions must be determined by the end user.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

Liquid. Contains no preservative.

Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

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