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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

1DS-1A6, monoclonal

species reactivity

human

technique(s)

western blot: suitable

isotype

IgG1κ

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

General description

Since the development of Green fluorescent protein’s (GFP) use as a protein tag in chimeric proteins, fluorescent proteins have become widely used and useful biotechnological tools. The isolation of Red Fluorescent proteins have opened up a broader color spectrum for use in research. These proteins can be fused with a wide variety of proteins at either the N- or C-terminus, and have been expressed as fluorescent markers in most cell types. These markers can be used as noninvasive markers in both living organisms and cells.
~ 28 kDa

Specificity

This antibody recognizes Red fluorescent protein.

Immunogen

Epitope: Unknown
Recombinant protein corresponding to sea anenome Red fluorescent protein.

Application

Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags

Physical form

Unpurified
Unpurified mouse monoclonal ascites with 0.05% sodium azide.

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Cos cell lysate with overexpression of RFP
Evaluated by Western Blot in Cos cell lysate with overexpression of RFP.

Western Blot Analysis: 1:1,000 dilution of this antibody detected Red fluorescent protein on 10 µg of Cos cell lysate with overexpression of RFP.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1


Certificates of Analysis (COA)

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