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MAB1254

Anti-Chymase Antibody, clone B7

clone B7, 2.4 mg/mL, Chemicon®

Synonym(s):

Mast Cell Protease I

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Key Documents

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Product Name

Anti-Chymase Antibody, clone B7, clone B7, 2.4 mg/mL, Chemicon®

biological source

mouse

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

B7, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

concentration

2.4 mg/mL

technique(s)

dot blot: suitable
immunohistochemistry: suitable

isotype

IgG1κ

suitability

not suitable for Western blot

NCBI accession no.

NM_001836.2

UniProt accession no.

P23946

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... CMA1(1215)

Application

Research Category
Inflammation & Immunology
Research Sub Category
Inflammation & Autoimmune Mechanisms
Anti-Chymase Antibody, clone B7 is an antibody against Chymase for use in DB, IH.
Dot blotting: 0.5-1.0 μg/mL.

Immunohistochemistry: 0.5-1.0 μg/mL. For optimal results, tissues or cytospin preparations should be fixed in Carnoy′s Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue.

Not recommended for use on Western blots.

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Reacts with human mast cell chymase.

Analysis Note

Control
POSITIVE CONTROL: lung, heart, skin, placenta.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Mast cell chymase (EC 3.4.21.39), a member of the granzyme subfamily of the S1 family of peptidases, cleaves C-terminal to Phenylalanine, Tyrosine, Tryptophan, and Leucine residues (in order of preference). Chymase is stored in Mast cell granules within the cell and is the major secreated Mast cell protease. Secreated Mast cell chymase is believed to be involved in extracellular matrix degradation, vasoactive peptide generation, and regulation of gland secreation.

Immunogen

Chymase purified from human skin.

Physical form

Format: Purified
Purified by protein G affinity chromatography. Liquid in PBS with 20% glycerol, pH 7.4.

Preparation Note

Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt. Antibody may also be stored at -20ºC in undiluted aliquots.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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M Yamada et al.
Kidney international, 59(4), 1374-1381 (2001-03-22)
Mast cells (MCs) are known to participate in various types of chronic disease, but their role in chronic renal rejection is poorly understood. Recently, distinct phenotypes of MCs have been described in humans by the demonstration of one protease, chymase.
Guo-Du Wang et al.
American journal of physiology. Gastrointestinal and liver physiology, 307(7), G719-G731 (2014-08-26)
Mast cells express the substance P (SP) neurokinin 1 receptor and the calcitonin gene-related peptide (CGRP) receptor in guinea pig and human small intestine. Enzyme-linked immunoassay showed that activation of intramural afferents by antidromic electrical stimulation or by capsaicin released

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