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Merck
CN

MAB1522

Anti-Actin Antibody, smooth muscle γ & α actin,

CHEMICON®, mouse monoclonal, CGA7

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
CGA7, monoclonal
Species reactivity:
human, rat, chicken, rabbit, monkey
Application:
IHC (p), WB
Citations:
30

Key Documents

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Product Name

Anti-Actin Antibody, smooth muscle γ & α actin, clone CGA7, ascites fluid, clone CGA7, Chemicon®

Quality Level

100

biological source

mouse

antibody form

ascites fluid

clone

CGA7, monoclonal

species reactivity

human, rat, chicken, rabbit, monkey

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable

isotype

IgG2a

NCBI accession no.

NM_001613.1

UniProt accession no.

P62736, P63267

shipped in

dry ice

target post-translational modification

unmodified

Immunogen

Chicken gizzard actin
Epitope: smooth muscle gamma & alpha actin

Application

Detect Actin using this Anti-Actin Antibody, smooth muscle γ & α actin, clone CGA7 validated for use in WB, IH, IH(P).
Western Blot: 1:1000 (approximately 43 kDa)

Immunohistochemistry (paraffin, formalin, deparaffinized methacarn-fixed tissue: 1:10,000 or alcohol fixation)

Optimal dilutions must be determined by the end user.

Biochem/physiol Actions

Antibody recognizes actin isotypes alpha and gamma of smooth muscle cells, but not skeletal or cardiac muscle cells. Reactive with myoepithelium, but nonreactive with other mesenchymal and all epithelial cells. The antibody labels eiomyosarcomas and leiomyomas but does not react with other neoplasms such as carcinomas or lymphoma.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A Kojima et al.
Virchows Archiv : an international journal of pathology, 434(5), 413-422 (1999-07-02)
We examined the expression of smooth muscle cytoskeleton in spindle-shaped cells in the capsule of hepatocellular carcinoma (HCC) and the septa of liver cirrhosis (LC). Serial sections of livers resected from 11 patients were stained with monoclonal antibodies against vimentin
Evidence for specific TRPM8 expression in human prostate secretory epithelial cells: functional androgen receptor requirement.
Bidaux, G; Roudbaraki, M; Merle, C; Crepin, A; Delcourt, P; Slomianny, C; Thebault et al.
Endocrine-Related Cancer null
Receptor-operated Ca2+ entry mediated by TRPC3/TRPC6 proteins in rat prostate smooth muscle (PS1) cell line.
S Thebault, A Zholos, A Enfissi, C Slomianny, E Dewailly, M Roudbaraki, J Parys, Natalia Prevarskaya
Journal of Cellular Physiology null
Brian J Leibowitz et al.
Nature communications, 5, 3494-3494 (2014-03-19)
The role of bone marrow (BM) and BM-derived cells in radiation-induced acute gastrointestinal (GI) syndrome is controversial. Here we use bone marrow transplantation (BMT), total body irradiation (TBI) and abdominal irradiation (ABI) models to demonstrate a very limited, if any
Mary E Donohoe et al.
Molecular cell, 25(1), 43-56 (2007-01-16)
In mammals, inactivation of one X chromosome in the female equalizes gene dosages between XX females and XY males. Two noncoding loci, Tsix and Xite, together regulate X chromosome fate by controlling homologous chromosome pairing, counting, and mutually exclusive choice.
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