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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
5A3.2, monoclonal
Application:
IHC, WB
Citations:
231
Quality Segment
biological source
mouse
conjugate
unconjugated
antibody form
culture supernatant
antibody product type
primary antibodies
clone
5A3.2, monoclonal
species reactivity
chicken, monkey, rat
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... POU4F1(5457)
General description
46 kDa
Immunogen
Amino acids 186-224 of Brn-3a fused to the T7 gene 10 protein (Xiang et al., 1995).
Epitope: POU-domain protein
Application
Anti-Brn-3a Antibody, POU-domain protein, clone 5A3.2 detects level of Brn-3a & has been published & validated for use in IH & WB.
Immunohistochemistry: 1:5-1:125
Western blot: 1:50-1:100
Optimal working dilutions must be determined by end user.
Western blot: 1:50-1:100
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Recognizes Brn-3a. Shows no reactivity to Brn-3b or Brn-3c by Western blot. Showed no reactivity to Brn-3a knock-out mice.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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