MAB1614
Anti-Kinesin Antibody, heavy chain, a.a.420-445, clone H2
clone H2, Chemicon®, from mouse
Synonym(s):
Anti-HEL-S-61, Anti-KINH, Anti-KNS, Anti-KNS1, Anti-UKHC
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
H2, monoclonal
Application:
ELISA, ICC, IHC, IP, RIA, WB
Citations:
66
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
H2, monoclonal
species reactivity
sea urchin, rat, chicken, squid, human, mouse
species reactivity (predicted by homology)
mammals
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, radioimmunoassay: suitable, western blot: suitable
isotype
IgG2b
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... KIF5B(3799)
General description
124 kDa
Kinesins are molecular motors used for intracellular transport and trafficking. They move along microtubule cables powered by the hydrolysis of ATP, thus kinesins are ATPases. The active movement of kinesins supports several cellular functions including mitosis, meiosis and transport of cargo such as axonal transport.
Immunogen
Bovine brain kinesin.
Epitope: a.a.420-445
Application
Detect Kinesin using this Anti-Kinesin Antibody, heavy chain, a.a.420-445, clone H2 validated for use in ELISA, IC, IH, IP, FUNC, RIA & WB.
Immunohistochemistry:
A previous lot was used on paraformaldehyde fixed tissue. Also works for EM.
Immunocytochemistry:
A previous lot of this antibody was used in Immunocytochemistry.
Immunoprecipitation:
A previous lot of this antibody was used in Immunoprecipitation.
EIA/RIA:
A previous lot of this antibody was used in RIA.
Immunoblotting: recognizes 124kDa KHC and its proteolytic fragments.
Inhibition of kinesin mediated transport {Brady, et al PNAS 87(3):1061-1065, 1990.
Optimal working dilutions must be determined by the end user.
A previous lot was used on paraformaldehyde fixed tissue. Also works for EM.
Immunocytochemistry:
A previous lot of this antibody was used in Immunocytochemistry.
Immunoprecipitation:
A previous lot of this antibody was used in Immunoprecipitation.
EIA/RIA:
A previous lot of this antibody was used in RIA.
Immunoblotting: recognizes 124kDa KHC and its proteolytic fragments.
Inhibition of kinesin mediated transport {Brady, et al PNAS 87(3):1061-1065, 1990.
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
Biochem/physiol Actions
Reacts with the heavy chain of kinesin near the N-terminus.
Physical form
Format: Purified
Protein A purified
Purified mouse monoclonal IgG2b in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl with 0.1% sodium azide.
Preparation Note
Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
Analysis Note
Control
Brain tissue
Brain tissue
Routinely evaluated by Western Blot on Mouse Brain lysates.
Western Blot Analysis:
1:500 dilution of this lot detected KINESIN (HEAVY CHAIN) on 10 μg of Mouse Brain lysates.
Western Blot Analysis:
1:500 dilution of this lot detected KINESIN (HEAVY CHAIN) on 10 μg of Mouse Brain lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Storage Class
12 - Non Combustible Liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Opposing microtubule motors drive robust nuclear dynamics in developing muscle cells.
Wilson, MH; Holzbaur, EL
Journal of Cell Science null
T Lynne Blasius et al.
PloS one, 8(9), e76081-e76081 (2013-10-08)
Kinesin motors drive the long-distance anterograde transport of cellular components along microtubule tracks. Kinesin-dependent transport plays a critical role in neurogenesis and neuronal function due to the large distance separating the soma and nerve terminal. The fate of kinesin motors
Muhammad Saad Yousuf et al.
eNeuro, 6(2) (2019-04-09)
Multiple sclerosis (MS) is an autoimmune, demyelinating disease of the central nervous system. Patients with MS typically present with visual, motor, and sensory deficits. However, an additional complication of MS in large subset of patients is neuropathic pain. To study
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAB1614 | 04053252727566 |