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UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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Product Name

Anti-Vimentin Antibody, clone LN-6, culture supernatant, clone LN-6, Chemicon®

biological source

mouse

Quality Level

100

antibody form

culture supernatant

antibody product type

primary antibodies

clone

LN-6, monoclonal

mol wt

calculated mol wt 53.65 kDa
observed mol wt ~N/A kDa

species reactivity

human, mouse

packaging

antibody small pack of 1

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable (paraffin)

isotype

IgM

epitope sequence

Unknown

NCBI accession no.

NM_003380.2

UniProt accession no.

P08670

shipped in

dry ice

storage temp.

-10 to -25°C

target post-translational modification

unmodified

Gene Information

human ... VIM(7431)

General description

Vimentin is a class III intermediate filament protein (homopolymers) found in various non-epithelial cells including mesenchymal cells and fibroblasts. In many cells of mesenchymal origin, it is one of the most prominent phosphoproteins, especially during cell division when enhanced phosphorylation of vimentin corresponds with the reorganization of vimentin filaments.

Specificity

Reacts with human mesenchymal cells and derived sarcomas. Reacts with an epitope of the cytoskeletal protein vimentin but unlike other vimentin antibodies, does not react with human lymphoid cells or derived malignancies. Reactive with endothelium, muscle, fibroblasts, melanocytes, peripheral nerve, sertoli cells, kidney mesangial cells and tubules, osteoblasts and periosteum, non-hematopoietic tumors of mesenchymal origin (all sarcomas), meningiomas and melanomas.

Immunogen

Nuclei from adult human thymic biopsy specimens.

Application

Anti-Vimentin Antibody, clone LN-6 detects level of Vimentin & has been published & validated for use in IH(P).
Immunohistochemistry on B5 fixed, paraffin embedded tissue sections.

Target description

57-60 kDa

Physical form

Liquid tissue culture supernatant containing 0.02% sodium azide.

Reconstitution

Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Hyperplastic lymph nodes.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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