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MAB1691

Sigma-Aldrich

Anti-Troponin I Antibody, a.a. 186-192, clone C5

clone C5, Chemicon®, from mouse

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eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

clone

C5, monoclonal

species reactivity

frog, mouse, chicken, fish, human, bovine, rabbit

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

isotype

IgG2b

NCBI accession no.

NM_000363.4

UniProt accession no.

P19429

shipped in

wet ice

target post-translational modification

unmodified

Specificity

Troponin I. Reacts with Troponin I from heart and skeletal muscle.

Immunogen

Bovine cardiac Troponin I.
Epitope: a.a. 186-192

Application

Detect Troponin I using this Anti-Troponin I Antibody, clone C5 validated for use in ELISA & WB.
Immunohistochemistry. The antibody is not recommended for use on parafin sections.

Troponin sandwich ELISA (for labeling) with MAB3438.

Western blotting. Recognizes a band at approxinmately 22.5 kDa corresponding to troponin I.

Troponin I purification

Optimal working dilutions must be determined by the end user.
Research Category
Metabolism
Research Sub Category
Muscle Physiology

Physical form

Format: Purified
Purified immunoglobulin from Protein A Sepharose. Liquid in PBS pH 7.4 with 0.1% sodium azide as a preservative.

Storage and Stability

Maintain refrigerated at +2-8°C for up to 6 months.

Analysis Note

Control
POSITIVE CONTROL: heart or skeletal muscle.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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pH-responsive titratable inotropic performance of histidine-modified cardiac troponin I.
Palpant, NJ; Houang, EM; Sham, YY; Metzger, JM
Biophysical Journal null
Cardioprotective effect of nicorandil, a mitochondrial ATP-sensitive potassium channel opener, prolongs survival in HSPB5 R120G transgenic mice.
Sanbe, A; Marunouchi, T; Yamauchi, J; Tanonaka, K; Nishigori, H; Tanoue, A
Testing null
Differential effects of a green tea-derived polyphenol (-)-epigallocatechin-3-gallate on the acidosis-induced decrease in the Ca(2+) sensitivity of cardiac and skeletal muscle.
Ying-Ming Liou,Shih-Chang Kuo,Shih-Rong Hsieh
Pflugers Archiv : European Journal of Physiology null
Human neonatal cardiovascular progenitors: unlocking the secret to regenerative ability.
Fuentes, TI; Appleby, N; Tsay, E; Martinez, JJ; Bailey, L; Hasaniya, N; Kearns-Jonker, M
Testing null
Christopher Jackman et al.
Acta biomaterialia, 78, 98-110 (2018-08-08)
The field of cardiac tissue engineering has developed rapidly, but structural and functional immaturity of engineered heart tissues hinder their widespread use. Here, we show that a combination of low-rate (0.2 Hz) contractile activity and thyroid hormone (T3) supplementation significantly promote
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