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Merck
CN

MAB1924

Anti-Laminin α5 Antibody, clone 4C7

ascites fluid, clone 4C7, Chemicon®

Synonym(s):

Anti-AA408762, Anti-AI853660, Anti-AA408760, Anti-laminin-511, Anti-mKIAA0533

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
4C7, monoclonal
Application:
ELISA, IHC, IP
Citations:
25
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biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

4C7, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable

isotype

IgG2a

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... LAMA2(3908)

Application

Anti-Laminin α5 Antibody, clone 4C7 is an antibody against Laminin α5 for use in ELISA, IH & IP.
ELISA (50% maximal binding to human laminin): >1:11,000.

Immunofluorescence - tissue staining pattern is most consistent with that observed for laminin alpha5 in the mouse(Miner et al., 1997)

Affinity chromatography

Immunoprecipitation

Does not work for Western blotting

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Reacts with the globular domain of the alpha5 chain of intact laminin and blocks the neurite stimulating activity of laminin. This antibody was originally thought to recognize laminin alpha 1 or A, but further characterization has confirmed its specificity for the laminin alpha 5 chain [see Tiger, C.F. (1997) J. Biol. Chem. 272: 28590].

Physical form

UnPurified mouse ascites containing no preservatives.

Analysis Note

Control
Developing striated muscle tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany


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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jamie L Maciaszek et al.
Biophysical journal, 102(5), 1137-1143 (2012-03-13)
Collapse and sudden death in physical training are the most serious complications of sickle cell trait (SCT). There is evidence that erythrocytes in SCT patients aggregate during strenuous exercise, likely because of adhesive interactions with the extracellular matrix (ECM) and
Giuseppe Mazza et al.
Scientific reports, 7(1), 5534-5534 (2017-07-19)
The development of human liver scaffolds retaining their 3-dimensional structure and extra-cellular matrix (ECM) composition is essential for the advancement of liver tissue engineering. We report the design and validation of a new methodology for the rapid and accurate production
Elena Daniele et al.
BMJ open ophthalmology, 7(1) (2022-09-27)
Recent clinical studies have shown that the transplantation of functional retinal pigment epithelium (RPE) cells can prevent the onset of RPE degeneration in age-related macular degeneration. This study aimed to investigate the potential of human amniotic membrane (hAM) as a



Global Trade Item Number

SKUGTIN
MAB192404053252360947