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MAB1940

Anti-Fibronectin Antibody

Name: Anti-Fibronectin Antibody
Brand: MM

CHEMICON^®, mouse monoclonal, DH1

Synonym(s):

Anti-CIG, Anti-ED-B, Anti-FINC, Anti-FN, Anti-FNZ, Anti-GFND, Anti-GFND2, Anti-LETS, Anti-MSF, Anti-SMDCF

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About This Item

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

Clone:

DH1, monoclonal

Species reactivity:

rat, guinea pig, human, rabbit

Application:

IHC, WB

Citations:

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Properties

Product Name

Anti-Fibronectin Antibody, cellular, clone DH1, clone DH1, Chemicon^®, from mouse

biological source

mouse

Quality Segment

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

DH1, monoclonal

species reactivity

rat, guinea pig, human, rabbit

manufacturer/tradename

Chemicon^®

technique(s)

immunohistochemistry: suitable, western blot: suitable

isotype

IgG1

NCBI accession no.

NM_002026.2, NM_054034.2, NM_212474.1, NM_212475.1, NM_212476.1, NM_212478.1, NM_212482.1

UniProt accession no.

P02751

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... FN1(2335)
rabbit ... Fn1(100328589)
rat ... Fn1(25661)

Description

Immunogen

Epitope: cellular

Purified fibronectin from Human A8387 fibrosarcoma cells.

Application

Detect Fibronectin using this Anti-Fibronectin Antibody, cellular, clone DH1 validated for use in WB, IH.

Immunoblotting at 1:1,000

Indirect immunohistochemistry at 1:200-1:400

A 1-hour fixation with 3-3.5% paraformaldehyde in 0.1 M phosphate buffer pH 7.4 is suggested. Shows some reactivity on paraffin sections depending on fixation conditions and time (Latvala et al., 1996).

Final working dilutions must be determined by end user.

Biochem/physiol Actions

Specific for the extra domain of cellular fibronectin (EDA sequence) and thus recognizes only the cellular fibronectin.

Physical form

Format: Purified

In PBS with 1% BSA and 0.1% sodium azide.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c