Anti-Laminin-5 Antibody, clone P3E4

Epiligrin, the major component of human keratinocyte extracellular matrix, serves as the preferred integrin ligand for alpha 3 beta 1 in plasma membranes and focal adhesions, and colocalizes with alpha 6 beta 4 in hemidesmosomes. {Domloge-Hultsch, et al (1992) J Clin Invest 90(4):1628-1633}. Major glycoprotein of epidermal basement membrane, consisting of three disulphide bonded subunits of 170, 145 and 135 kD. Epiligrin is the major ligand for alpha3/beta1 integrin, is particularly prominent in the lamina lucida of the skin and is absent in patients with lethal junctional epidermolysis bullosa. Today, epiligrin is identical to laminin 5, also called BM600, nicein, and kalinin. Laminin 5 (epiligrin; Carter et al, 1991 Cell 65(4):599-610) as the primary ligand in epithelial BMs that mediates both cell anchorage (adhesion without migration) via integrin a6b4 in homeostatic tissue and cell migration via integrin a3b1 in wound repair.

The laminin-5 isoform (nicein, epiligrin, and kalinin) is abundant in transitional epithelium, stratified squamous epithelia, lung mucosa, and other epithelial glands (Kallunki et al., 1992; Stahl et al., 1997). Laminin-5 is a heterotrimer consisting of alpha3, beta3, and gamma2 subunits that associate via large helical regions to produce a cruciform-shaped molecule (Rousselle et al., 1991 J. Cell Biol. 114: 567-576; Baker et al., 1996 J. Cell Sci. 109: 2509-2520). Laminin-5 is synthesized initially as a 460-kD molecule that undergoes specific processing to a smaller form after being secreted into the extracellular matrix (Marinkovich et al., 1992 J. Biol. Chem. 267: 17900-17906; Vailly et al., 1994 Eur. J. Biochem. 219: 209-218; Matsui et al., 1995 J. Biol. Chem. 270: 23496-23503). The size reduction is a result of processing the 3 and 2 subunits from 190-200 to 160 kD and from 155 to 105 kD, respectively (Marinkovich et al., 1992; Vailly et al., 1994; Matsui et al., 1995). {Goldfinger, LE (1998) J Cell Biol 141(1):255-265}.

Human keratinocytes

Immunohistochemistry: for use on acetone fixed tissue

Immunocytochemistry

Immunoblotting: (non-reducing)

Immunoprecipitation

ELISA

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

This Anti-Laminin-5 Antibody, clone P3E4 is validated for use in ELISA, IP, WB, IC, IH for the detection of Laminin-5.

Human epiligrin (laminin 5)

Format: Purified

Please see datasheet for lot-specific information.

Store at +2 to 8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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