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Merck
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MAB2148-C

Anti-PECAM-1 Antibody, clone P2B1 (Ascites Free)

clone P2B1, 1 mg/mL, from mouse

Synonym(s):

Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA′, PECA1, CD31

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Key Documents

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Product Name

Anti-PECAM-1 Antibody, clone P2B1 (Ascites Free), clone P2B1, 1 mg/mL, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

P2B1, monoclonal

species reactivity

human

concentration

1 mg/mL

technique(s)

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG1κ

NCBI accession no.

NP_000433

UniProt accession no.

P16284

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... PECAM1(5175)

Related Categories

Analysis Note

Evaluated by Flow Cytometry in human PBMCs.

Flow Cytometry Analysis: 1 µg of this antibody detected PECAM-1 in 1X10E6 human PBMCs.

Application

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected PECAM-1 in pluripotent cells (Boyd, N. L., et al. (2007). Exp Biol Med. 232(6):833-843.).

Immunoprecipitation Analysis: A representative lot detected PECAM-1 by immunoprecipitation.

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected PECAM-1 in astrocytoma tissue sections (Bronger, H., et al. (2005). Cancer Res. 65(24):11419-28.).

ELISA Analysis: A representative lot of from an independent laboratory detected PECAM-1 in a panel of CD31 mutant cell lines (Newton, J. P, et al. (1997) J. Biol. Chem., 272: 20555-63.).

Immunofluorescence Analysis: A representative lot from an independent laboratory detected PECAM-1 in astrocytoma tissue sections (Bronger, H., et al. (2005). Cancer Res. 65(24):11419-28.).
Research Category
Cell Structure
Research Sub Category
ECM Proteins
This Anti-PECAM-1 Antibody, clone P2B1 (Ascites Free) is validated for use in flow cytometry, ICC, IP, IHC, ELISA & immunofluorescence for the detection of PECAM-1.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

PECAM-1 is a type I integral membrane glycoprotein, and a member of the immunoglobulin superfamily of cell surface receptors. PECAM-1 is constitutively expressed on the surface of endothelial cells where it is concentrated at cell junctions. PECAM-1 is also expressed at low levels on all leukocytes and platelets. Homotypic interaction of CD31 is important in cell-cell interaction, cell-matrix interaction and signal transduction.
~80 kDa calculated

Immunogen

Whole cells expressing human PECAM-1.

Other Notes

Replaces: 04-1074, MAB2148

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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