MAB2259
Anti-Cholesterol-24 Hydroxylase Antibody, clone1A7
clone 1A7, from mouse
Synonym(s):
Cytochrome P450 46A1, cholesterol 24-hydroxylase, cytochrome P450, family 46, cytochrome P450, family 46, subfamily A, polypeptide 1, cytochrome P450, subfamily 46 (cholesterol 24-hydroxylase)
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
1A7, monoclonal
Application:
ICC, IHC, IP, WB
Citations:
3
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
1A7, monoclonal
species reactivity
mouse
species reactivity (predicted by homology)
rat (based on 100% sequence homology), human (based on 100% sequence homology), rabbit (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CYP46A1(10858)
General description
In enzymology, a cholesterol 24-hydroxylase (EC 1.14.13.98) is an enzyme that catalyzes the chemical reaction:
cholesterol + NADPH + H+ + O2 --> (24S)-24-hydroxycholesterol + NADP+ + H2O. Cholesterol 24-hydroxylase, a cytochrome P450 (CYP46A1), is expressed at 100-fold higher levels in the brain than in the liver. This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. Studies indicate that cholesterol 24-hydroxylase constitutes a major tissue-specific pathway for cholesterol turnover in the brain (Lund, 2003).
cholesterol + NADPH + H+ + O2 --> (24S)-24-hydroxycholesterol + NADP+ + H2O. Cholesterol 24-hydroxylase, a cytochrome P450 (CYP46A1), is expressed at 100-fold higher levels in the brain than in the liver. This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. Studies indicate that cholesterol 24-hydroxylase constitutes a major tissue-specific pathway for cholesterol turnover in the brain (Lund, 2003).
~ 54 kDa
Immunogen
Epitope: unknown
His-tagged recombinant protein corresponding to human cholesterol-24 hydroxylase.
Application
Detect Cholesterol-24 Hydroxylase using this Anti-Cholesterol-24 Hydroxylase Antibody, clone1A7 validated for use in WB, IP, IC, IH.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Signaling Neuroscience
Signaling Neuroscience
Biochem/physiol Actions
This antibody recognizes cholesterol-24 hydroxylase.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Mouse brain lysate
Mouse brain lysate
Evaluated by Western Blot in Cholesterol-24 hydroxylase mouse brain lysate.
Western Blot Analysis: 1 µg/ml of this antibody detected Cholesterol-24 hydroxylase on 10 µg of Cholesterol-24 hydroxylase mouse brain lysate.
Western Blot Analysis: 1 µg/ml of this antibody detected Cholesterol-24 hydroxylase on 10 µg of Cholesterol-24 hydroxylase mouse brain lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Related Content
Fuxin Lu et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 41(2), 312-323 (2020-03-15)
The major pathway of brain cholesterol turnover relies on its hydroxylation into 24S-hydroxycholesterol (24S-HC) using brain-specific cytochrome P450 46A1 (CYP46A1). 24S-HC produced exclusively in the brain normally traverses the blood-brain barrier to enter the circulation to the liver for excretion;
Ahmed Haider et al.
Science translational medicine, 14(665), eadc9967-eadc9967 (2022-10-06)
Alterations in brain cholesterol homeostasis have been broadly implicated in neurological disorders. Notwithstanding the complexity by which cholesterol biology is governed in the mammalian brain, excess neuronal cholesterol is primarily eliminated by metabolic clearance via cytochrome P450 46A1 (CYP46A1). No
H Benson Peng et al.
Biopharmaceutics & drug disposition, 42(8), 372-388 (2021-07-06)
Age, hypercholesterolemia, and vitamin D deficiency are risk factors that increase the brain accumulation of pathogenic β-amyloid peptides (40 and 42), precursors leading to Alzheimer's disease (AD) in humans. The relative changes accompanying aging, high cholesterol, and/or treatment of calcitriol
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAB2259 | 04053252467332 |