62 kDa

The activity of the main glutamate transporter in the CNS, Excitatory amino acid transporter 2 (also known as GLT1), can be regulated by protein kinase C (PKC). It is known that activation of PKC by phorbol esters promotes the clathrin-dependent internalization of the transporter, followed by its lysosomal degradation. However, the molecular mechanisms that link PKC activation and the internalization of the excitatory amino acid transporter 2 are not fully understood. The internalization process may play a role in the ubiquitylation of lysine residues located in the C-terminal tail of the excitatory amino acid transporter 2. Exposure to PMA increases the ubiquitylation of the excitatory amino acid transporter 2 in transfected cells and in the rat brain, and this ubiquitylated excitatory amino acid transporter 2 accumulates in the intracellular compartment. The activation of PKC induces the ubiquitylation of these C-terminal lysine residues in the Excitatory amino acid transporter 2 and this modification mediates the interaction of the transporter with the endocytic machinery.

Epitope: C-terminus

Linear peptide corresponding to the C-terminus of human Excitatory amino acid transporter 2.

Research Category
Neuroscience

Research Sub Category
Signaling Neuroscience

This Anti-Excitatory amino acid transporter 2 Antibody, clone G6 is validated for use in WB, IH, IC for the detection of Excitatory amino acid transporter 2.

Western Blot Analysis: 0.5 µg/ml from a previous lot detected Excitatory amino acid transporter 2 on 10 µg of human brain tissue lysate.

This antibody recognizes the C-terminus of Excitatory amino acid transporter 2.

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Mouse brain tissue lysate

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected Excitatory amino acid transporter 2 on 10 µg of mouse brain tissue lysate.

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