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Merck
CN

MAB3045

Anti-Connexin 35/36 Antibody, clone 8F6.2

clone 8F6.2, Chemicon®, from mouse

Synonym(s):

Cx35/36

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
8F6.2, monoclonal
Application:
IHC
Citations:
45

Key Documents

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biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

8F6.2, monoclonal

species reactivity

monkey, fish, rabbit

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable

isotype

IgG1

UniProt accession no.

Q8JFD6

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

rabbit ... Gjd2(100341036)

Application

Detect Connexin 35/36 using this Anti-Connexin 35/36 Antibody, clone 8F6.2 validated for use in IH.

Biochem/physiol Actions

Recognizes perch Connexin 35. Does not cross-react with Connexin 34.7, a very closely related protein. Also recognizes rabbit and macaque Connexin 36, the gene homologue of Connexin 35. Labels gap junctions in retinas of perch, rabbit and macaque in immunohistochemistry.

Physical form

Format: Purified
Purified immunoglobulin. Liquid in 0.1M Citrate buffer with 50% glycerol, also contains 0.1% Sodium Azide as a preservative

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MAB3044

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Adam C Miller et al.
eLife, 6 (2017-05-23)
Neural network function is based upon the patterns and types of connections made between neurons. Neuronal synapses are adhesions specialized for communication and they come in two types, chemical and electrical. Communication at chemical synapses occurs via neurotransmitter release whereas
Yuan-Hao Lee et al.
Bioscience and bioengineering (Boston, Mass.), 4(4), 78-84 (2018-12-01)
Calcium signals act as a ubiquitous secondary messenger in regulating many body functions. The detection of calcium microdomain signals is greatly facilitated by the existence of biomarker-targeted fluorescent probes. In this study, SRRF (super-resolution radial fluctuations) algorithm were used to
Adenosine and dopamine receptors coregulate photoreceptor coupling via gap junction phosphorylation in mouse retina.
Li, H; Zhang, Z; Blackburn, MR; Wang, SW; Ribelayga, CP; O'Brien, J
The Journal of Neuroscience null
Distribution of the gap junction protein connexin 35 in the central nervous system of developing zebrafish larvae.
Jabeen, S; Thirumalai, V
Frontiers in Neural Circuits null
J E Rash et al.
Journal of neurocytology, 33(1), 131-151 (2004-06-03)
Combined confocal microscopy and freeze-fracture replica immunogold labeling (FRIL) were used to examine the connexin identity at electrical synapses in goldfish brain and rat retina, and to test for "co-localization" vs. "close proximity" of connexins to other functionally interacting proteins
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