MAB318-AF488
Anti-Tyrosine Hydroxylase Antibody, clone LNC1, Alexa Fluor™ 488 Conjugate
clone LNC1, from mouse, ALEXA FLUOR™ 488
Synonym(s):
Tyrosine 3-monooxygenase, Tyrosine Hydroxylase, Tyrosine 3-hydroxylase, TH
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
ALEXA FLUOR™ 488
Clone:
LNC1, monoclonal
Application:
ICC
Citations:
6
biological source
mouse
Quality Level
conjugate
ALEXA FLUOR™ 488
antibody form
purified antibody
antibody product type
primary antibodies
clone
LNC1, monoclonal
species reactivity
human, chicken, mouse, frog, zebrafish, rat
technique(s)
immunocytochemistry: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
General description
Tyrosine 3-monooxygenase (EC 1.14.16.2; UniProt P07101; also known as Dystonia 14, TH, Tyrosine 3-hydroxylase) is encoded by the TH (also known as DYT14, DYT5b, TYH) gene (Gene ID 7054) in human. Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. TH enzymatic activity requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. Six isoforms of human TH exist as a result of alternative splicing (UniProt P07101-1 through P07101-6).
~59-63 kDa observed
Immunogen
Epitope: On the outside of the regulatory N-terminus of tyrosine hydroxylase.
Tyrosine Hydroxylase purified from PC12 cells.
Application
The unconjugated antibody (Cat. No. MAB318) is shown to be suitable also for Western blotting, immunohistochemistry, and immunoprecipitation applications.
Anti-Tyrosine Hydroxylase Antibody, clone LNC1, Alexa Fluor™ 488 Conjugate is an antibody against Tyrosine Hydroxylase for use in Immunocytochemistry.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Developmental Neuroscience
Biochem/physiol Actions
Recognizes an epitope on the outside of the regulatory N-terminus of tyrosine hydroxylase. Does not react with dopamine-beta-hydroxylase, phenylalanine hydroxylase, trytophan hydroxylase, dehydropteridine reductase, sepiapterin reductase, or phenethanolamine-N-methyl transferase (PNMT) by Western blotting using the unconjugated antibody (Cat. No. MAB318).
Physical form
Protein A purified
Purified mouse antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Immunocytochemistry in rat cortical neurons.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Tyrosine Hydroxylase in rat cortical neurons.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Tyrosine Hydroxylase in rat cortical neurons.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Kara A Fulton et al.
eLife, 10 (2021-05-14)
A dense reconstruction of neuronal synaptic connectivity typically requires high-resolution 3D electron microscopy (EM) data, but EM data alone lacks functional information about neurons and synapses. One approach to augment structural EM datasets is with the fluorescent immunohistochemical (IHC) localization
Alterations in the social-conditioned place preference and density of dopaminergic neurons in the ventral tegmental area in Clsnt2-KO mice.
Rozhkova, et al.
Vavilovskii zhurnal genetiki i selektsii, 27, 177-184 (2023)
Lei Tang et al.
Nature communications, 14(1), 7497-7497 (2023-11-19)
The degenerative process in Parkinson's disease (PD) causes a progressive loss of dopaminergic neurons (DaNs) in the nigrostriatal system. Resolving the differences in neuronal susceptibility warrants an amenable PD model that, in comparison to post-mortem human specimens, controls for environmental
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAB318-AF488 | 04055977301168 |