Oligodendrocytes and astrocytes are derived from common precursor cells, glioblasts (Sommer, 1981). O-Antigens are sulfatides, which function as differentiation markers on the surface of oligodendrocytes of the central nervous system. O4 is formed postnatally (Schachner, 1981) and is a marker for cell bodies and processes of oligodendrocytes types I and II (hairy eyeball type). During oligodendrocyte differentiation, O4 occurs in pro-oligodendrocytes, but not in O-2A-progenitor cells. O4 occurs from day 3 onwards in cell cultures of embryonic mouse brain. Anti-O4 can be used in myelination, demyelination and remyelination studies and in regeneration experiments.

Homogenate of white matter of corpus callosum from bovine brain.

Anti-O4 Antibody, clone 81, Alexa Fluor^™488 Conjugate detects level of O4 & has been published & validated for use in ICC.

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

This antibody recognizes Oligodendrocyte marker O4. Also reacts with certain galactolipids in sperm.

Precipitation followed by ion exchange purification

Purified mouse monoclonal IgM conjugated to Alexa Fluor^™ 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Control
Undifferentiated rat oligodendrocyte precursor cells

Evaluated by Immunocytochemistry in undifferentiated rat oligodendrocyte precursor cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected O4 in undifferentiated rat oligodendrocyte precursor cells.

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