Anti-Enkephalin Antibody, clone NOC1

Leu5-enkephalin conjugated to BSA.

Detect Enkephalin using this Anti-Enkephalin Antibody, clone NOC1 validated for use in IC, IH.

Immunohistochemistry: 1:100-1:400 on 10 μm cryostat sections of rat and guinea-pig brain stem using fluorescently labled secondary antibodies for detection (Cuello, 1984; MacMillan and Cuello, 1986).

Immunocytochemistry: 1:300 on primary cultures of rat striatal and hippocampal neurons using a Cy2 or Cy3 conjugated secondary antibody for detection (Saudou, 1998).

Radioimmunoassay (Cuello, 1984)

Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Neuroinflammation & Pain

Hormones & Receptors

Met5- and Leu5-enkephalin. Displays about 40% cross-reactivity with C-terminal extended Met-enkephalin hexapeptides and 7% cross-reactivity with the extended heptapeptide (-Arg-Phe-OH) but does not recognize pro-enkephalin or other endogenous peptides (Cuello, 1984). By immunohistochemistry the antibody lables all sites that are known to exppress enkephalin and shows no cross-reactivity to beta-endorphin or dynorphin (Cuello, 1984; MacMillan and Cuello, 1986).

Lyophilized Supernatant, preservative free.

Lyophilized antibody can be kept at 2-8°C for up to 3 months and should be kept at -20°C for long term storage. Reconstitute with 100μL sterile DI water. Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

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