MAB3584
Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-1H5
ascites fluid, clone 2HP-1H5, Chemicon®
Synonym(s):
HP1a, Antigen p25, Chromobox Protein Homolog 5
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
2HP-1H5, monoclonal
Application:
ELISA, ICC, IHC
Citations:
41
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
2HP-1H5, monoclonal
species reactivity
mouse, human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable
isotype
IgG1κ
suitability
not suitable for Western blot
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... CBX5(23468)
General description
Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication.
Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.
Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.
Immunogen
Recombinant mouse HP1a.
Application
Immunocytochemistry: 1:500-1:5,000
Immunohistochemistry: 1:500-1:5,000
ELISA: 1:500-1:5,000
Western blot: not recommended. For Western blot use MAB3446.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry: 1:500-1:5,000
ELISA: 1:500-1:5,000
Western blot: not recommended. For Western blot use MAB3446.
Optimal working dilutions must be determined by the end user.
Use Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-1H5 (mouse monoclonal antibody) validated in ELISA, ICC, IHC to detect Heterochromatin Protein-1 α also known as HP1a, Antigen p25, Chromobox Protein Homolog 5.
Biochem/physiol Actions
Reacts with Heterochromatin protein-1 alpha (HP1alpha) between amino acids 67 and 119.
Physical form
Liquid ascites no preservative.
Analysis Note
Control
Positive Control: HeLa cells, 3T3 cells , F9 cells, mouse embryos
Positive Control: HeLa cells, 3T3 cells , F9 cells, mouse embryos
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Still not finding the right product?
Try our Product Selector Tool to narrow your options
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Targeting polycomb to pericentric heterochromatin in embryonic stem cells reveals a role for H2AK119u1 in PRC2 recruitment.
Cooper, S; Dienstbier, M; Hassan, R; Schermelleh, L; Sharif, J; Blackledge, NP; De Marco et al.
Cell Reports null
Putative Epimutagens in Maternal Peripheral and Cord Blood Samples Identified Using Human Induced Pluripotent Stem Cells.
Arai, Y; Hayakawa, K; Arai, D; Ito, R; Iwasaki, Y; Saito, K; Akutsu, K; Takatori et al.
BioMed Research International null
DNA methylation promotes Aurora-B-driven phosphorylation of histone H3 in chromosomal subdomains.
Monier, K; Mouradian, S; Sullivan, KF
Journal of Cell Science null
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAB3584 | 04053252463624 |