Skip to Content
Merck
CN

MAB360

Sigma-Aldrich

Anti-Glial Fibrillary Acidic Protein Antibody

CHEMICON®, mouse monoclonal, GA5

Synonym(s):

GFAP

Sign Into View Organizational & Contract Pricing

Select a Size

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5, ascites fluid, clone GA5, Chemicon®

biological source

mouse

Quality Level

100

antibody form

ascites fluid

antibody product type

primary antibodies

clone

GA5, monoclonal

species reactivity

rat, human, mouse, rabbit, chicken, pig, bovine

packaging

antibody small pack of 25 μL

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_002055.2

UniProt accession no.

P14136

shipped in

ambient

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GFAP(2670)

Related Categories

General description

Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.
~ 51 kDa

Immunogen

Purified GFAP from porcine spinal cord.

Application

Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 detects level of Glial Fibrillary Acidic Protein & has been published & validated for use in IC, IH, IH(P) & WB with more than 65 product citations.
Immunocytochemistry:
A previous lot was used on cultured mammalian cells.

Immunohistochemistry(paraffin):
1:400-1:800 dilution from a previous lot was used on alcohol-fixed paraffin embedded sections of rat brain (cerebrum or cerebellum) and human brain.

Immunoblotting: A 1:1000 dilution of a previous lot was used.

Optimal working dilutions must be determined by the end user

Biochem/physiol Actions

Glial fibrillar acidic protein. On western blots of extracts from a human glioma cell line (U33CG/343MG), MAB360 recognizes a band at approximately 51 kDa corresponding to GFAP (Debus, 1983). By immunohistochemistry it recognizes astrocytes and Bergmann glia cells, glioma and glial cell derived tumors. Shows no cross-reactivity with vimentin.
Reactivity with other species has not been determined.

Physical form

Unpurified mouse monoclonal IgG1 liquid in buffer containing 15 mM sodium azide.

Analysis Note

Control
Positive Control: Cultured astrocytes, brain tissue (white matter), spinal chord, retina
Routinely evaluated by Western Blot on Mouse brain lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected GFAP on 10 μg of Mouse brain lysates.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
Replaces: 04-1031; 04-1062

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Lateral fluid percussion: model of traumatic brain injury in mice.
Alder, J; Fujioka, W; Lifshitz, J; Crockett, DP; Thakker-Varia, S
Journal of Visualized Experiments null
Y Jiang et al.
Brain research, 1332, 100-109 (2010-03-30)
Recent work suggests that diabetes affects processing of peripheral, spinal and supraspinal signals in the spinal cord. However, there is little evidence for spinal cord lesions that would account for alterations in behavioral responses induced by experimental diabetes. Therefore, we
Adenosine kinase as a target for therapeutic antisense strategies in epilepsy.
Theofilas, P; Brar, S; Stewart, KA; Shen, HY; Sandau, US; Poulsen, D; Boison, D
Epilepsia null
Jan Gunnar Sørbø et al.
FEBS letters, 581(25), 4884-4890 (2007-09-28)
Aquaporin-4 (AQP4) has been reported to be upregulated post-partum in pregnancy and in early lung development. Several technical challenges exist in measuring AQP4 protein levels, among them sensitivity to detergent solubilization, sample heating and gel composition. Here we have optimized
Biochip?laser cell deposition system to assess polarized axonal growth from single neurons and neuron?glia pairs in microchannels with novel asymmetrical geometries.
Pirlo, RK; Sweeney, AJ; Ringeisen, BR; Kindy, M; Gao, BZ
Biomicrofluidics null
View All Related Papers

Protocols

Tips and Techniques for Troubleshooting Immunohistochemistry (IHC)

Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.

Related Content

默克高品质抗体_ELISA试剂盒-默克生命科学

抗体的质量决定了整个免疫检测的成败， 是在选择抗体时首选的考虑因素。基于Sigma®、Chemicon®、Upstate®、Mil l ipore®、 Calbiochem®和Novagen®等子品牌的强强联合，默克在创新的 免疫原设计、免疫、挑选、筛选和验证等方面有着数十年的经验， 创造出许多被广泛使用的抗体，如4G10，Neun，组蛋白修饰等抗 体都是相关领域的“金标”抗体。 我们的技术和科研背景使默克成为高质量抗体的主要设计者和生产 者，而不仅仅是经销商。毋庸置疑，我们的每个小瓶中的抗体都包 含着大量的科学研究成果。

PRODUCTO REGULADO POR LA SECRETARÍA DE SALUD

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service